FDG - Galactosidase Assay Kit
Referentie CA083
Formaat : 500assays
Merk : Canvax Biotech
FDG – Galactosidase Assay Kit
Fluorometric assay kit for sensitive detection of β-galactosidase activity in cells.
FDG β-Galactosidase Assay Kit is a fluorometric assay for measuring β-galactosidase activity in living cells. It uses fluorescein di-β-D-galactopyranoside (FDG) as a substrate that produces a fluorescent signal upon cleavage.
This kit provides sensitive and quantitative detection of enzyme activity and is suitable for reporter gene studies and cell-based assays.
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Categories: Cell Based Assays, Other Assays & Compounds
Detailed information:
Advantages & Features
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High sensitivity: femtogram-level detection.
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Convenient: ready-to-use reagents and buffers.
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Robust: optimized for transfected cell lysates.
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Fluorometric detection: Ex/Em = 490/525 nm.
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Large capacity: 500 assays per kit.
Specifications
| Specification | Description |
|---|---|
| Detection | Fluorometric (Ex/Em = 490/525 nm) |
| Sensitivity | Femtogram-level β-galactosidase |
| Assay type | Cell lysates (transfected with lacZ) |
| Assay capacity | 500 assays |
| Includes | Buffers, enzyme, FDG substrate, controls |
Includes
– 55 mL Reaction Buffer
– 25 mL Lysis Buffer
– 25 mL Stop solution
– 20 μL β-galactosidase enzyme (0.1 mg/mL)
– 500 μL β-Mercaptoethanol
– 150 μL FDG solution (10 mM)
Download documentation
Applications
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Reporter gene assays (lacZ).
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Measurement of β-galactosidase activity in transfected cell lysates.
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Transfection efficiency monitoring.
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Gene expression analysis.
Tables & Figures
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Quality Control
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Each batch validated for reproducibility and sensitivity.
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Strict lot-to-lot quality assurance testing.
Advice
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Protect FDG substrate from light.
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Ensure complete lysis of cells for maximum signal.
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: Upon receipt, store the kit at -20 ºC. β-galactosidase substrate must be protected from light.
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
Synonym(s)
Beta-galactosidase assay kit, FDG β-gal assay, Fluorescein di-β-D-galactopyranoside assay, β-gal activity assay, Fluorogenic β-galactosidase assay
Also known as:
- Spanish: Kit de ensayo de β-galactosidasa, Ensayo FDG, Ensayo fluorogénico de β-galactosidasa
- French: Kit d’analyse β-galactosidase, Test FDG, Essai fluorogénique β-galactosidase
- German: β-Galactosidase-Assaykit, FDG-Test, Fluorogener β-Galactosidase-Assay
- Italian: Kit saggio β-galattosidasi, Saggio FDG, Saggio fluorogenico β-galattosidasi
FAQs
Q: What is the detection method?
A: Fluorometric, Ex/Em = 490/525 nm.
Q: How many assays can be performed?
A: Up to 500 per kit.
Q: What is the sensitivity?
A: Detects β-galactosidase at femtogram levels.