K2212-100 | Glucose Uptake Fluorometric Assay Kit Clinisciences

Description

Glucose metabolism is a process that converts ingested glucose into energy, which is the main source of energy supply in living organisms. Studies have shown that glucose metabolism is closely related to many diseases such as cancer, diabetes and obesity, so it is important to monitor glucose uptake.

The 2-NBDG Glucose Uptake Assay Kit is a kit for the detection of glucose uptake by the fluorescent probe 2-NBDG. 2-NBDG is a fluorescently labeled glucose analogue that is transported into cells by glucose transporter (GLUT) like glucose. Once 2-NBDG is uptaken in cells, it is phosphorylated at the C-6 position to produce 2-NBDG-6-phosphate, which is well retained in cells. Compared to other glucose trackers (2-DG or FDG), 2-NBDG allows in situ detection of glucose uptake at the single-cell level.

This kit provides a sensitive, rapid and non-radioactive assay to detect glucose uptake. At the same time, the kit provides phloretin, a GLUT1 inhibitor that can significantly inhibit glucose uptake, as a positive control. For 96-well plates, using 100 μL of working solution per well, the kit can be used at least 500 times.

Figure 1: The efficiency of this kit for detecting glucose uptake

Quality Control

Protocol

Components and Storage

Components	K2212-500 T
2-NBDG	500 μL
PI (1000x)	250 μL
Phloretin (100x)	100 μL
5x Assay Buffer	20 mL
Store the kit at -20°C, stable for 1 year. 2-NBDG, PI (1000x) and Phloretin (100x) should be stored away from light.

Glucose Uptake Fluorometric Assay Kit

Referentie K2212-100

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Description

Quality Control

Components and Storage