HIS Tag Antibody
Referentie OAAB06612
Formaat : 200ul
Merk : Aviva Systems Biology
HIS Tag Antibody (OAAB06612)
| Datasheets/Manuals | Printable datasheet for HIS Tag Antibody (OAAB06612) |
|---|
| Predicted Species Reactivity | Human |
|---|---|
| Product Format | Liquid PBS with 0.09% (W/V) sodium azide. |
| Clonality | Monoclonal |
| Clone | 6AT18 |
| Isotype | IgG1 |
| Host | Mouse |
| Application | WB |
| Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Immunogen | Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal antibody. |
| Purification | This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Concentration | Approximately 0.5mg/ml. Actual concentration varies with each lot. |
| Application Info | WB: 1:2000 WB: 1:1,000 |
| Intended Use | HIS Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Description of Target | Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells. |
|---|
