RayBio® G-Series Human Apoptosis Antibody Array 1 Kit. Detects 43 Human Apoptotic Factors. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
Human Apoptosis Array G1
Human Apoptosis Array G1
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Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 43 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Apoptosis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
bad
| bax
| bcl-2
| bcl-w
| BID
|
BIM
| Caspase-3
| Caspase-8
| CD40 (TNFRSF5)
| CD40 Ligand (TNFSF5)
|
cIAP-2
| Cytochrome C | DR6 (TNFRSF21)
| Fas (TNFRSF6/Apo-1)
| Fas Ligand (TNFSF6)
|
HSP27
| HSP60
| HSP70
| HTRA2
| IGF-1
|
IGF-2
| IGFBP-1
| IGFBP-2
| IGFBP-3
| IGFBP-4
|
IGFBP-5
| IGFBP-6
| IGF-1 R
| livin
| p21
|
p27
| p53
| SMAC
| Survivin (BIRC5)
| TNF RI (TNFRSF1A)
|
TNF RII (TNFRSF1B)
| TNF alpha
| TNF beta (TNFSF1B)
| TRAIL R1 (TNFRSF10A/DR4)
| TRAIL R2 (TNFRSF10B/DR5)
|
TRAIL R3 (TNFRSF10C)
| TRAIL R4 (TNFRSF10D)
| XIAP
| ||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Apoptosis Antibody Array G1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Protease Inhibitor Cocktail
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-AAH-APO-G1-4.gal)
GAL (GAL-AAH-APO-G1-8.gal)
Excel (AAH-APO-G1_Analysis_Tool.xls)
citations
Combined oncolytic virotherapy gold nanoparticles as synergistic immunotherapy agent in breast cancer control
- Species:
- Human
- Sample Type:
- MCF-7 cell
- Year:
- 2023
- Journal Name:
- Scientific reports
Targeting cathepsin L in the regulation of apoptosis in peripheral T-cell lymphoma
- Species:
- Human
- Sample Type:
- Jurkat cell
- Year:
- 2023
- Journal Name:
- Molecular & Cellular Toxicology
Epigenetic silencing of miR-137 induces drug resistance and chromosomal instability by targeting AURKA in multiple myeloma
- Species:
- Human
- Sample Type:
- Cell Lysate (H929 and mir-137 overexpressing cells)
- Year:
- 2017
- Journal Name:
- Leukemia
- PubMed ID:
- 27857131
The influence of simulated microgravity on proliferation and apoptosis in U251 glioma cells.
- Species:
- Human
- Sample Type:
- Cell Lysate (U251 glioma cells); Cell Lysate (U251MG cell culture); HeLa cell
- Year:
- 2017
- Journal Name:
- In Vitro Cellular and Developmental Biology Animals
- PubMed ID:
- 28707224
WAP four-disulfide core domain protein 2 gene (WFDC2) is a target of estrogen in ovarian cancer cells.
- Species:
- Human
- Sample Type:
- Cell Lysate ( HO8910-NA and HO8910-209 cells)
- Year:
- 2016
- Journal Name:
- Journal of ovarian research
Isoledene from Mesua ferrea oleo-gum resin induces apoptosis in HCT116 cells through ROS-mediated modulation of multiple proteins in the apoptotic pathways: A mechanistic study
- Species:
- Human
- Sample Type:
- Cell Lysate (HCT116 colorectal carcinoma cells)
- Year:
- 2016
- Journal Name:
- Toxicology Letters
- PubMed ID:
- 27268964
Molecular mechanisms responsible for programmed cell death-inducing attributes of terpenes from Mesua ferrea stem bark towards human colorectal carcinoma HCT 116 cells
- Species:
- Human
- Sample Type:
- Cell Lysate (HCT116 colorectal carcinoma cells)
- Year:
- 2016
- Journal Name:
- Journal of Applied Biomedicine
Myricitrin attenuates endothelial cell apoptosis to prevent atherosclerosis: An insight into PI3K/Akt activation and STAT3 signaling pathways
- Species:
- Human
- Sample Type:
- Cell Lysate (HUVEC Cells)
- Year:
- 2015
- Journal Name:
- Vascular Pharmacology
- PubMed ID:
- 25849952
BARF1 gene silencing triggers caspase-dependent mitochondrial apoptosis in Epstein-Barr virus-positive malignant cells
- Species:
- Human
- Sample Type:
- Cell Lysate (AG876 and Hone-Akata cells transfected with BARF1 siRNA); uterus
- Year:
- 2015
- Journal Name:
- Journal of Biosciences
- PubMed ID:
- 25740140
Expression profile of apoptotic and proliferative proteins in hypoxic HUVEC treated with statins
- Species:
- Human
- Sample Type:
- Cell Lysate (HUVEC Cells)
- Year:
- 2014
- Journal Name:
- International Journal of Oncology
- PubMed ID:
- 25434456