Human CD62E/E-selectin Antibody : APC
Referentie OASB00976
Formaat : 100tests
Merk : Aviva Systems Biology
Human CD62E/E-selectin Antibody : APC (OASB00976)
| Datasheets/Manuals | Printable datasheet for Human CD62E/E-selectin Antibody : APC (OASB00976) |
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| Tested Species Reactivity | Human |
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| Predicted Species Reactivity | Human |
| Clonality | Monoclonal |
| Clone | 1.2B6 |
| Isotype | IgG1 |
| Host | Mouse |
| Conjugation | APC |
| Application | FC |
| Additional Information | Description: CD62E represents the 97 kDa type I transmembrane glycoprotein E-selectin, also known as endothelial-leukocyte adhesion molecule-1 (ELAM-1). It is expressed on cytokine-stimulated endothelium, weakly on unactivated endothelium, and is absent on leukocytes. Like other selectins, CD62E is an adhesion molecule that contributes to the initial attachment and rolling of leukocytes on endothelial surfaces, a prerequisite for leukocyte extravasation into tissues. CD62P is a 140 kDa membrane glycoprotein formerly known as platelet activation-dependent granule membrane protein (PADGEM) or GMP-140. It is expressed on platelets, megakaryocytes, and epithelium. It is thought to mediate the adhesive interactions of neutrophils and monocytes with endothelium in inflammatory responses and of activated platelets to neutrophils and monocytes in hemostasis. The monoclonal antibody 1.2B6 recognizes a common epitope shared by CD62E and CD62P. |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | TNF stimulated human endothelial cells |
| Concentration | Lot specific |
| Specificity | CD62E/CD62P |
| Characterization | To insure lot to lot consistency, each batch of product is tested by Immunocytochemistry to conform withthe characteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified (UNLB) antibody <= 1 ug/106 cells Fluorescein conjugate 10 uL/106 cells BIOT conjugate 10 uL/106 cells R-phycoerythrin conjugate 10 uL/106 cells Allophycocyanin 10 uL/106 cells Spectral Red conjugate 10 uL/106 cells |
| Application Info | Flow cytometry, Immunohistochemistry (frozen sections), Immunocytochemistry, Immunoprecipitation, Western blotting, ELISA, Inhibition of activity |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.0. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of phosphate buffered saline containing0.1% PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE), allophycocyanin (APC), are supplied as 100 tests in 1.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD) conjugate is supplied 100 tests in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored asdirected. |
| Reference | 1. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd,eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford 2. Gearing, A.J.H., and W. Newman. 1993. Immunol. Today 14:506 3. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte AntigensFacts Book, 2nd Edition, CD62E Section, Academic Press, New York, p. 295 4. Tedder, T.F., D.A. Steeber, A. Chen, and P. Engel. 1995. FASEB J. 9:866. |
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| Gene Symbol | SELE, SELP |
| Gene Full Name | E-selectin |
| Alias Symbols | ELAM, ESEL, CD62E, ELAM1, LECAM2 |
| NCBI Gene Id | 6401; 6403; 397508 |
| Protein Name | E-selectin |
| Description of Target | The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. |
| Uniprot ID | P16581, P16109, P98110 |
| Protein Accession # | NP_000441.2 |
| Nucleotide Accession # | NM_000450.2 |
