RayBio® Human MMP-2 ELISA Kit for cell culture supernatants, heparin treated plasma, and serum samples. EDTA and Citrate are not recommended.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human MMP-2 ELISA Kit Standard Curve
Human MMP-2 ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 3,500 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 3.5 ng/ml - 800 ng/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 3, -7, -8, -10, -12, -13, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 5 - 50 fold |
| Accession Number | P08253 |
| Gene Id | 4313 |
| Gene Symbols | MMP2, CLG4A |
| Protein Name / Synonyms | 72 kDa type IV collagenase (EC 3.4.24.24) (72 kDa gelatinase) (Gelatinase A) (Matrix metalloproteinase-2) (MMP-2) (TBE-1) [Cleaved into: PEX] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
documents
citations
Alcohol consumption modulates Candida albicans-induced oral carcinogenesis and progression.
- Species:
- Human
- Sample Type:
- Ca9-22 cell
- Year:
- 2023
- Journal Name:
- Journal of Oral Biosciences
The Inflammatory Profile Orchestrated by Inducible Nitric Oxide Synthase in Systemic Lupus Erythematosus
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Journal of Personalized Medicine
Hemimycale Arabica Induced Non-Cytotoxic Anti-Migratory Activity in Hepatocellular Carcinoma In Vitro
- Species:
- Human
- Sample Type:
- HepG2 cell
- Year:
- 2022
- Journal Name:
- Asian Pacific Journal of Cancer Prevention
The FoxP1 gene regulates lung function, production of matrix metalloproteinases and inflammatory mediators, and viability of lung epithelia
- Species:
- Human
- Sample Type:
- BEAS-2B cell
- Year:
- 2022
- Journal Name:
- Respiratory Research
Tamoxifen/raloxifene loaded liposomes for oral treatment of breast cancer
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2020
Biomarkers of Skin Graft Healing in Venous Leg Ulcers
- Species:
- Human
- Sample Type:
- wound fluid
- Year:
- 2022
- Journal Name:
- Acta dermato-venereologica
Matrix metalloproteinase activation by free neutrophil elastase contributes to bronchiectasis progression in early cystic fibrosis
- Species:
- Human
- Sample Type:
- BAL Fluid (CF and control patients)
- Year:
- 2015
- Journal Name:
- European Respiratory Journal
- PubMed ID:
- 25929954
The role of HPV gene expression and selected cellular MiRNAs in lung cancer development
- Species:
- Human
- Sample Type:
- Tissue Lysate (homogenized tumor tissue)
- Year:
- 2020
- Journal Name:
- Microbial Pathogenesis
Markers of increased cardiovascular risk in patients with chronic kidney disease
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2014
- Journal Name:
- Lipids in Health and Disease
Inhibition of aldose reductase prevents angiogenesis in vitro and in vivo.
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2011
- Journal Name:
- Angiogenesis
- PubMed ID:
- 21409599
