K1072-50 | HyperScript™ First-Strand cDNA Synthesis Kit Clinisciences

Product Citation

1. Bing Li, Ge Lou, et al. "Repression of lncRNA PART1 attenuates ovarian cancer cell viability, migration and invasion through the miR-503-5p/FOXK1 axis." BMC Cancer. 2022 Jan 31;22(1):124. PMID: 35100978
2. Hongrong Wang, Shasha Song, et al. "Long non-coding RNA HOTAIR knockdown alleviates lipopolysaccharide-induced acute respiratory distress syndrome and the associated inflammatory response by modulating the microRNA-30a-5p/PDE7A axis." Exp Ther Med. 2021 Oct;22(4):1160. PMID: 34504605
3. Yu-Qing Pan, Min Niu, et al. "Effect of MT2A on apoptosis and proliferation in HL60 cells." Int J Med Sci. 2021 Jun 4;18(13):2910-2919. PMID: 34220318
4. Yonglei Liu, Lixia Wang, et al. "Ramelteon Ameliorates LPS-Induced Hyperpermeability of the Blood-Brain Barrier (BBB) by Activating Nrf2." Inflammation. 2021 Oct;44(5):1750-1761. PMID: 33876343

Description

The HyperScript™ First-Strand cDNA Synthesis Kit is based on HyperScript™ Reverse Transcriptase (Cat. No. K1071) to synthesize first-strand cDNA from Total RNA or Poly(A)+ RNA. HyperScript™ Reverse Transcriptase is a new enzyme obtained through genetic engineering based on M-MLV (RNase H-) Reverse Transcriptase. In comparison, HyperScript™ Reverse Transcriptase reduces RNase H activity and increases thermal stability. HyperScript™ Reverse Transcriptase can withstand higher reaction temperatures and is suitable for reverse transcription of RNA templates with complex secondary structures. In addition, HyperScript™ Reverse Transcriptase enhances the affinity to the template, suitable for the reverse transcription of a small number of templates and low copy genes, and can generate cDNA up to 12.3 kb.

The HyperScript™ First-Strand cDNA Synthesis Kit contains all the components needed to synthesize the first-strand cDNA and provides two cDNA synthesis primers, Random Primers and Oligo (dT)23VN. You can choose Random Primers, Oligo (dT)23VN or gene-specific primers as reverse transcription primers according to the needs of the experiment. The synthesized first-strand cDNA products can be used in subsequent experiments such as PCR amplification and qPCR reaction.

Quality Control

Components and Storage

Components	20 rxn (20 μL reaction)	50 rxn (20 μL reaction)	100 rxn (20 μL reaction)
HyperScript™ Reverse Transcriptase (200 U/μL)	20 μL	50 μL	100 μL
5X First-Strand Buffer	80 μL	200 μL	400 μL
RNase Inhibitor, Murine(40 U/μL)	20 μL	50 μL	100 μL
10 mM dNTP Mixture	20 μL	50 μL	100 μL
RNase Free ddH₂O	1 mL	1 mL	1 mL x 2
Random Primers (50 μM)	20 μL	50 μL	100 μL
Oligo (dT) ₂₃VN (50 μM)	20 μL	50 μL	100 μL
Store the components at -20°C.

Features

Efficient HyperScript™ Reverse Transcriptase for RNA templates containing secondary structures

Synthesis of first-strand cDNA up to 12.3 kb

Detects template starting amounts as low as 1 pg

Oligo (dT)23VN primers have stronger template anchoring ability and higher reverse transcription efficiency than Oligo (dT)18

Synthesized first-strand cDNA can be used for experiments such as PCR amplification and qPCR reaction

Storage

Store the components at -20°C.

HyperScript™ First-Strand cDNA Synthesis Kit

Referentie K1072-50

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