IgM (Biotin)
Referentie I1905-01S-1mg
Formaat : 1mg
Merk : US Biological
I1905-01S IgM (Biotin)
Clone Type
PolyclonalHost
goatSource
ratIsotype
IgGGrade
Affinity PurifiedApplications
E IHC WBCrossreactivity
RtShipping Temp
Blue IceStorage Temp
4°C (-20°C Glycerol)
Applications:
Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested.
Recommended Dilutions:
ELISA: 1:20,000-1:100,000
Immunohistochemistry: 1:1000-1:5000
Western Blot: 1:2000-1:10,000
Optimal dilution determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Applications
Product Type: Pab|Isotype: IgG|Host: goat|Source: rat|Concentration: ~1mg/ml (UV280) (after reconstitution)|Form: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitute with 1ml sterile ddH2O.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Rat IgM mu chain.|Specificity: Recognizes Rat IgM mu chain. Assay by IEP resulted in a single precipitin arc against anti-biotin, anti-goat serum, rat IgM and rat serum. No reaction was observed against rat IgA or rat IgG.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Rat IgM mu chain.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitute with 1ml sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes Rat IgM mu chain. Assay by IEP resulted in a single precipitin arc against anti-biotin, anti-goat serum, rat IgM and rat serum. No reaction was observed against rat IgA or rat IgG.

