Lipase Activity Fluorometric Assay Kit III

Lipase is an enzyme that catalyzes fats (lipids) hydrolysis and is a subclass of the esterases. Lipase plays important roles in the digestion, transport and processing of dietary lipids (e.g. oils, triglycerides, fats) in most living organisms. In human digestive system, pancreatic lipase is the key enzyme that converts triglycerides to monoglycerides and free fatty acids. In the damaged pancreas, lipase levels can rise 5 to 10-fold within 24 to 48 hours. The Lipase Activity Fluorometric Assay Kit III provides a highly sensitive, easy and fast way for detection of lipase activity in various samples based on fluorometric method. In the assay, lipase in the sample hydrolyzes a specific substrate to produce the methylresorufin, which can be detected at Em/Ex = 529/600 nm by fluorometric method. The kit is suited for high throughput screening assay of lipase activity and can measure lipase activity as low as 0.1 mU/well.