MARK2 Recombinant Antibody
Referentie bsm-54447R
Formaat : 100uL
Merk : Bioss
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MARK2 (1F10)) Monoclonal Antibody, Unconjugated (bsm-54447R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MARK2 (1F10)) Monoclonal Antibody, Unconjugated (bsm-54447R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54447r |
| Product Name | MARK2 Recombinant Antibody |
| Applications | WB, FCM, IHC-P, IHC-F, IF(IHC-P) |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein near the C-terminus of human MARK2. |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 2011 |
| Swiss Prot | Q7KZI7 |
| Subcellular location | Cytoplasm, Cell membrane, Cytoskeleton |
| Synonyms | Serine/threonine-protein kinase MARK2; ELKL motif kinase 1; MAP/microtubule affinity-regulating kinase 2; EMK-1; Par-1b; Par1b; MARK2; EMK1 |
| Background | MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens]. EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule.stability and cancer. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1 called MARK2. These isoforms were characterized by the presence of a 162-bp alternative exon that gave rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be coexpressed in a number of selected cell lines and tissue samples. Human MARK2 was shown to be encoded by a single mRNA ubiquitously expressed. This transcription variant includes the alternative exon in the coding region and therefore codes for a longer protein. Multiple splice variants exist for this protein. |
| Application Dilution | |
| WB | 1:500-1000 |
| FCM | FCM1:100-200 |
| IHC-P | IHC-P1:50-200 |
| IHC-F | IHC-F1:400-800 |
| IF(IHC-P) | IFIHC-P1:100-500 |