OABB01586 | MCL1 Antibody Clinisciences

SARS-CoV-2 Spike RBD Nanobody, Biotin conjugated Recombinant Antibody [A1] (OACA12530)

Datasheets/Manuals	Printable datasheet for SARS-CoV-2 Spike RBD Nanobody, Biotin conjugated (OACA12530)

Product Info
Predicted Species Reactivity	SARS-CoV-2 / COVID-19 Virus
Product Format	Liquid 0.01M PBS with 0.03% Proclin300 and 50% glycerol
Clonality	Recombinant Monoclonal - Nanobody
Clone	A1
Isotype	VHH fusion with human IgG1 Fc
Conjugation	Biotin
Application	Enzyme-linked immunosorbent assay
Reconstitution and Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Immunogen	Recombinant Human Novel Coronavirus Spike glycoprotein(S) (319-541aa) (CSB-YP3324GMY1 and CSB-MP3324GMY1b1)
Purification	Affinity chromatography
Concentration	Varies by lot. See vial for concentration.
Application Info	ELISA: 1:10000~50000

Gene Symbol	S
Gene Full Name	surface glycoprotein
Alias Symbols	E2;GU280_gp02;Peplomer protein;spike glycoprotein;surface glycoprotein.
NCBI Gene Id	43740568
Protein Name	Spike glycoprotein
Description of Target	Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32221306, PubMed:32075877, PubMed:32155444). Binding to host NRP1 and NRP2 via C-terminal polybasic sequence enhances virion entry into host cell (PubMed:33082294, PubMed:33082293). This interaction may explain virus tropism of human olfactory epithelium cells, which express high level of NRP1 and NRP2 but low level of ACE2 (PubMed:33082293). The stalk domain of S contains three hinges, giving the head unexpected orientational freedom (PubMed:32817270). Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Can be alternatively processed by host furin (PubMed:32362314). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membrane fusion within endosomes.
Uniprot ID	P0DTC2