MMP13 ELISA Kit (Human)
Referentie OKEH00174
Formaat : 96Wells
Merk : Aviva Systems Biology
MMP13 ELISA Kit (Human) : 96 Wells (OKEH00174)
| Datasheets/Manuals | Printable datasheet for MMP13 ELISA Kit (Human) : 96 Wells (OKEH00174) |
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| COA Datasheet | Printable COA datasheet for OKEH00174 |
| Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology MMP13 ELISA Kit (Human) (OKEH00174) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP13 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP13 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MMP13 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.7% (n = 20) Mean Inter-assay CV%: < 9.8% (n = 20) | ||||||||||||||||||||||
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.039 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Human Collagenase 3 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | MMP13 |
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| Gene Full Name | matrix metallopeptidase 13 |
| Alias Symbols | CLG3, collagenase 3, MANDP1, matrix metalloproteinase 13 (collagenase 3), Matrix metalloproteinase-13, MDST, MMP-13. |
| NCBI Gene Id | 4322 |
| Protein Name | Collagenase 3 |
| Description of Target | Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion. |
| Uniprot ID | P45452 |
| Protein Accession # | NP_002418 |
| Nucleotide Accession # | NM_002427 |
| Protein Size (# AA) | 471 |
| Molecular Weight | 54 kDa |
