Quantibody® Mouse Cytokine Array 5 Kit. Detects 40 Mouse Cytokines. Suitable for all liquid sample types.
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Mouse Cytokine Array Q5
Mouse Cytokine Array Q5
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 40 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
bFGF
| BLC (CXCL13)
| CD30 Ligand (TNFSF8)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
|
Fas Ligand (TNFSF6)
| GCSF
| GM-CSF
| ICAM-1 (CD54)
| IFN-gamma
|
IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-2
| IL-3
| IL-4
|
IL-5
| IL-6
| IL-7
| IL-10
| IL-12 p40
|
IL-13
| IL-15
| IL-17A
| IL-21
| KC (CXCL1)
|
Leptin
| LIX
| MCP-1 (CCL2)
| MCP-5
| M-CSF
|
MIG (CXCL9)
| MIP-1 alpha (CCL3)
| MIP-1 gamma
| Platelet Factor 4 (CXCL4)
| RANTES (CCL5)
|
TARC (CCL17)
| I-309 (TCA-3/CCL1)
| TNF alpha
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Mouse Cytokine Array Q5 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAM-CYT-5.gal)
Excel (QAM-CYT-5_Analysis_Tool.xls)
citations
Use of novel structural features to identify urinary biomarkers during acute kidney injury that predict progression to chronic kidney disease
- Species:
- Mouse
- Sample Type:
- urine
- Year:
- 2023
- Journal Name:
- Bmc Nephrology
GAD1 Upregulation Programs Aggressive Features of Cancer Cell Metabolism in the Brain Metastatic Microenvironment
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Cancer associated fibroblasts and tumor cells in a transwell setup)
- Year:
- 2017
- Journal Name:
- Cancer Research
- PubMed ID:
- 28400476
Activities of Murine Peripheral Blood Lymphocytes Provide Immune Correlates That Predict Francisella tularensis Vaccine Efficacy
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Francisella infected bone marrow macrophages)
- Year:
- 2016
- Journal Name:
- Infection and Immunity
- PubMed ID:
- 26810039
Cardiac-restricted Overexpression of TRAF3 Interacting Protein 2 (TRAF3IP2) Results in Spontaneous Development of Myocardial Hypertrophy, Fibrosis, and Dysfunction
- Species:
- Mouse
- Sample Type:
- Tissue Lysate (Left Ventricular lysates in a cardiac stress model)
- Year:
- 2016
- Journal Name:
- The Journal of Biological Chemistry
- PubMed ID:
- 27466370
Correlates of Vaccine-Induced Protection against Mycobacterium tuberculosis Revealed in Comparative Analyses of Lymphocyte Populations
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Co cultures of bone marrow derived macrophates, infected with mycobaterium bovis)
- Year:
- 2015
- Journal Name:
- Clinical and Vaccine Immunology
- PubMed ID:
- 26269537
Chronic inflammation induces telomere dysfunction and accelerates ageing in mice
- Species:
- Mouse
- Sample Type:
- Conditioned Media (WT vs NFkb-/- mouse ear fibroblasts); Tissue Lysate (Frozen Liver Lysates)
- Year:
- 2014
- Journal Name:
- Nature Communications
Adiponectin fine-tuning of liver regeneration dynamics revealed through cellular network modeling
- Species:
- Mouse
- Sample Type:
- Serum; Tissue Lysate (Liver Lysate)
- Year:
- 2014
- Journal Name:
- Journal of Physiology
- PubMed ID:
- 25384784
Specific gut commensal flora locally alters T cell tuning to endogenous ligands.
- Species:
- Mouse
- Sample Type:
- Serum (model of autoimmune arthritis)
- Year:
- 2013
- Journal Name:
- Immunity
Interleukin-6 is essential for primary resistance to Francisella tularensis live vaccine strain infection.
- Species:
- Mouse
- Sample Type:
- Tissue Lysate (mouse lung, livers, and spleens in IL-6 KO mice)
- Year:
- 2013
- Journal Name:
- Infection and Immunity
- PubMed ID:
- 23230288
Donor Satellite Cell Engraftment is Significantly Augmented When the Host Niche is Preserved and Endogenous Satellite Cells are Incapacitated
- Species:
- Mouse
- Sample Type:
- Tissue Lysate (Tibialis anterior muscle lysates)
- Year:
- 2012
- Journal Name:
- Stem Cells