Mouse IgG (gamma chain) Antibody Alkaline Phosphatase
Referentie orb347565-1mg
Formaat : 1mg
Merk : Biorbyt
Mouse IgG (gamma chain) Antibody Alkaline Phosphatase
SKU: orb347565
Description
Research Area
Signal Transduction
Images & Validation
−
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:1,000 - 1:11,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Mouse IgG gamma heavy chain |
| Purity | Anti-Mouse IgG (gamma chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigen coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse or human heavy or light chain isotypes. |
| Conjugation | AP |
Storage & Handling
−| Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-Mouse IgG Antibody alkaline phosphatase conjugation, rabbit anti-Mouse IgG gamma alk phos conjugated Antibody
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)