Mouse Progesterone,PROG ELISA Kit

Referentie CSB-E05104m-96T

Formaat : 96T

Merk : Cusabio


CSB-E05104m
Size 96T,5×96T,10×96T

Product Details

Target Name
Progesterone,PROG
Abbreviation
PROG
Species
Mus musculus (Mouse)
Sample Types
serum, plasma
Detection Range
0.3 ng/mL-10 ng/mL
Sensitivity
0.2 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PROG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Sample

Serum(n=4)

1:1

Average %

96

Range %

92-104

1:2

Average %

93

Range %

91-97

1:4

Average %

102

Range %

98-106

1:8

Average %

87

Range %

82-94

Recovery

The recovery of mouse PROG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

94

90-97

EDTA plasma (n=4)

89

85-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

10

0.175

0.189

0.182

5

0.452

0.456

0.454

2.5

0.759

0.783

0.771

0.86

1.175

1.251

1.213

0.3

1.647

1.685

1.666

0

2.085

2.130

2.107

ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description

This Mouse PROG ELISA Kit was designed for the quantitative measurement of Mouse PROG protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.3 ng/mL-10 ng/mL and the sensitivity is 0.2 ng/mL.

Citations

  • Polystyrene Nanoplastics induced placental toxicology by activating Keap1-mediated ferroptosis via METTL3-dependent m6A methylation C Yujiao, L Yifan, Z Meng, L Shanshan, Y Yousheng,Cell biology and toxicology,2026
  • T Wu, Y Feng, Q Zhu, T Jiang, Y Gao, K Nie,Advanced Science,2026
  • M Wang, L Du, X Fu, W Shi, Y Su, L Li,Journal of Traditional Chinese Medicine,2026
  • H Khaleel, SM Thaker, HA Ali,Journal of Applied Science, Engineering, Technology, and Education,2025
  • Y Wu, Y Chen, S Wang, T Wu, J Zhang,Reproductive Toxicology,2026
  • L Zeng, X Huang, T Chen, Z Yu, C Huang, B Xia,Phytomedicine,2025
  • Y Ma, Z Jin, A Yang,Journal of Ovarian Research,2025
  • Q Zhou, X Li, N Wang, L Zhang, E Jiang, K Wang,Cell Death & Disease,2025
  • YL Ma, ZW Li, Z Wang, AJ Yang,Molecular Biology,2024
  • J Wen, Y Feng, L Xue, S Yuan, Q Chen, A Luo,Gut Microbes,2024

Customer Reviews and Q&A

Average Rating:
5.0 - 1 reviews

Sample type: Serum

Sample species: Mouse

Sample dilution: No dilution

Review: I used CSB-E05104m to test the serum of mice. Group od od- blank 1+ (x/C)^B concentration ave control C36 0.82 0.7843 24.54654803 0.47730038 1.187548202 C29 0.4774 0.4417 6.960100594 1.783379399 C32 0.6501 0.6144 14.1283102 0.858463521 C34 0.5804 0.5447 10.84184426 1.130691667 C35 0.5093 0.4...

By Anonymous

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