B8209-10mg | Nile Red [7385-67-3] Clinisciences

Nile Red, is a fluorescent stain specific for the detection of intracellular lipid droplets in hydrophobic environment, but show minimal fluorescence in aqueous media. Nile Red can be applied for staining intracellular lipids, lysosomal phospholipid inclusions and hydrophobic domains of proteins, and detected by fluorescence microscopy and flow cytofluorometry. In all organic solvents, Nile Red is intensely fluorescent, with colors ranging from golden yellow to red.

Reference:

1. Greenspan P, Mayer EP, Fowler SD. Nile red: a selective fluorescent stain for intracellular lipid droplets. Journal of Cell Biology, 1985, 100(3): 965-973.

Storage	Store at -20°C
M.Wt	318.37
Cas No.	7385-67-3
Formula	C₂₀H₁₈N₂O₂
Solubility	≥2.56 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O
Chemical Name	9-(diethylamino)-5H-benzoamphenoxazin-5-one
SDF	Download SDF
Canonical SMILES	CCN(C1=CC2=C(N=C3C4=CC=CC=C4C(C=C3O2)=O)C=C1)CC
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Cell experiment:[1]
Cell lines	Monkey aortic smooth muscle cells and mouse peritoneal macrophages, induced by acetylated low density lipoprotein
Reaction Conditions	100 ng/ml Nile Red
Applications	Better selectivity for cytoplasmic lipid droplets was obtained when the cells were viewed for yellow-gold fluorescence (excitation, 450-500 nm; emission, greater than 528 nm) rather than red fluorescence (excitation, 515-560 nm; emission, greater than 590 nm).
Note	The technical data provided above is for reference only.
	References: 1. Greenspan P, Mayer EP, Fowler SD. Nile red: a selective fluorescent stain for intracellular lipid droplets. Journal of Cell Biology, 1985, 100(3): 965-973.

Purity = 98.87%

Chemical structure

Nile Red [7385-67-3]

Referentie B8209-10mg

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Background

Chemical Properties

Protocol

Quality Control

Chemical structure

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