Oligo, Reverse Sequencing Primer M13 (16-mer), [5’-AAC AGC TAT GAC CAT G-3’]

Single-stranded oligonucleotide, chemically synthesized, suitable for DNA applications. This M13 primer is a reverse sequencing primer that enables sequencing inserts in M13mp and pUC vectors.

Special Labels: Biotin, Fluorescein, and Phosphate modifications can be included at the 5' or 3' site. For special labels add -B, -F, -P and -5, -3 to the catalog number.

Sequence:
5' AAC AGC TAT GAC CAT G 3'

Molecular Weight:
4874.2g/mol

GC Content:
43.8%

Tm:
46.6°C

Synthesis Scale:
50nmole
Synthesis scale refers to the amount of the 3'-terminus base that the multi-step synthesis starts with. Final yield is indicated on the lot-specific certificate of analysis.

Minimum Guaranteed Yield:
1.0 OD (A260nm) unit per mL reconstituted product
1 OD unit corresponds to ~33ug ssDNA

Storage & Stability:
Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.