600374 | Rat GM-CSF Clinisciences

Recombinant Active Protein

Cat. No.

600374

Unit

0.02 mg

Description

Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is hematopoietic factor produced by entothelial cells, monocytes, fibroblasts and T cells in response to a number of inflammatory mediators. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. Human and mouse GM-CSF show no cross-reactivity. Rat GM-CSF is produced in E.coli. Recombinant rat GM-CSF is a non-glycosylated protein, containing 128 amino acids, with a molecular weight of 14.6 kDa.

Spec Container

Datasheet

Format

Each vial contains 20 ug of lyophilized protein. Reconstitute with 0.2 ml sterile deionized water for a final concentration of 0.1 mg/ml.

Alternate Names

Granulocyte-macrophage colony-stimulating factor; GM-CSF; colony-stimulating factor; CSF; Csf2; Csfgm

Accession No.

P13109

14600 Da

Sequence

MAPTRSPNPV TRPWKHVDAI KEALSLLNDM RALENEKNED VDIISNEFSI QRPTCVQTRL KLYKQGLRGN LTKLNGALTM IASHYQTNCP PTPETDCEIE VTTFEDFIKN LKGFLFDIPF DCWKPVQK

Source

E. coli. Endotoxin level as measured by LAL is <0.05ng/ug or <0.5EU/ug.

Purity

Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Application Notes

The activity is determined by the dose-dependant proliferation of the mouse FDC-P1 cell line and is typically less than 100 pg/mL. Specific activity: 1 x 10e7 units/mg

Storage

Store at -20C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.

Images

For research use only, not for diagnostic or therapeutic procedures.

Rat GM-CSF

Referentie 600374

Merk : Abbiotec

Spec Container