Recombinant IFN alpha 1a (Interferon alpha 1a), Human, Animal-Free
Referentie orb1179087-5ug
Formaat : 5ug
Merk : Biorbyt
Recombinant IFN alpha 1a (Interferon alpha 1a), Human, AF
View GuideView Protocol
Recombinant IFN alpha 1a (Interferon alpha 1a), Human, Animal-Free
SKU: orb1179087
Description
Research Area
Infectious Disease & Virology; Immunology & Inflammation
Images & Validation
−| Tested Applications | Cell Culture, ELISA |
|---|
Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Origin | Human |
| Biological Activity | Measure by its ability to inhibit IL-8 secretion in human PBMCs in the presence of LPS. The ED50 for this effect is <1.12 μg /mL. |
| Reactivity | Human |
| Tag | His-tag at the N-terminus |
| Endotoxins | <0.1 EU per 1 μg of the protein by the LAL method. |
| Purity | >95% as determined by SDS-PAGE. Ni-NTA chromatography. |
| Protein Sequence | CDLPETHSLDNRRTLMLLAQMSRISPSSCLMDRHDFGFPQEEFDGNQFQKAPAISVLHELIQQIFNLFTTKDSSAAWDEDLLDKFCTELYQQLNDLEACVMQEERVGETPLMNADSILAVKKYFRRITLYLTEKKYSPCAWEVVRAEIMRSLSLSTNLQERLRRKE with polyhistidine tag at the N-terminus. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | The protein was lyophilized from a solution containing 1X PBS, pH 8.0. |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Leukocyte Interferon, IFNA2, B cell Interferon, Type I Interferon
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
ELISA
Enzyme-linked Immunosorbent Assay (EIA)