Topoisomerase II alpha (TOP2A) (C-term) Mouse Monoclonal Antibody [Clone ID: 1C5]

Specifications

Product Data
Clone Name	1C5
Applications	FC, IF, IHC, IP, WB
Recommended Dilution	Western blot: 0.1-0.5 µg/ml for chemiluminescence detection system. Immunoprecipitation: 1-10 µg/200-300 µg/ml of cell extract. Immunohoistochemistry on paraffin sections: Heat treatment is necessary; Microwave oven; 2 times for 10 minutes aech in 10 mM citrate buffer (pH 6.5). Immunocytochemistry: 1-10 µg/ml. Flow cytometry: 0.1-1 µg/ml (final contentration).
Reactivities	Human
Host	Mouse
Isotype	IgG2a
Clonality	Monoclonal
Immunogen	Recombinant human Topoisomerase II alpha protein corresponding to C-termional 182 aa
Specificity	This antibody detects 170 kDa of human Topo II alpha on Western blot with total cell lysate from human cell lines.
Formulation	PBS containing 50 % glycerol, pH 7.2. No preservative is contained. State: Azide Free State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein A agarose
Conjugation	Unconjugated
Storage	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Stability	Shelf life: One year from despatch.
Gene Name	topoisomerase (DNA) II alpha
Database Link	Entrez Gene 7153 Human UniProt ID P11388
Background	Topoisomerase II alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- and M-phases in transformed and developmentally regulated normal cells. Topoisomerase II alpha is also implicated in drug resistance of tumor cells.
Synonyms	TOP2, Top-2, DNA topoisomerase 2-alpha, DNA topoisomerase II alpha isozyme
Note	This product was originally produced by MBL International.
Reference Data