Bile Esculin Agar (BEA) - Selective solid media for microbiology

Bile Esculin Agar (BEA) - Selective solid media for microbiology


Bile Esculin Agar (or Enterococcal Agar or BEA) is prepared according to the formula specified by Swan, and subsequently evaluated by Facklam and Moody. It is a selective medium for the isolation of Enterococci and Group D Streptococci. It was concluded that the biliary esculin test was a reliable means of identifying enterococci and differentiating them from non-enterococci. This medium contains bile to inhibit the growth of gram-positive bacteria as well as many gram-negative bacteria. Sodium azide also suppresses the growth of gram-negative bacteria and Streptococci except those of group D. Enterococci hydrolyze esculin to esculetin, which forms a complex with ferric citrate to produce insoluble iron salts, causing the medium to turn brown-black or black. Enterococci therefore form small translucent colonies surrounded by a black halo.

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