Glucose Oxidase BioAssay™ Kit

Referentie 172368-100T

Formaat : 100Tests

Merk : US Biological

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Telefoonnummer : +1 850 650 7790


Shipping Temp
Blue Ice

Storage Temp
4°C/-20°C

Glucose oxidase catalyzes the oxidation of glucose from D-glucose to Dglucono-d-lactone. Physiologically, it aids in the breakdown of glucose into smaller metabolites. It is widely used in electrochemical glucose sensors designed for diabetes patients. Simple, direct and high throughput assays for measuring glucose oxidase activity find wide applications in research and drug discovery. The glucose oxidase assay kit uses a single Working Reagent that combines the glucose oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570nm or fluorescence intensity at lex/em=530/585nm is directly proportional to glucose oxidase activity in the sample.

Intended Use:
For quantitative determination of glucose oxidase activity and evaluation of drug effects on its metabolism.

Applications:
Direct Assays: glucose oxidase activity in cell lysate, culture medium and other biological samples.
Drug Discovery/Pharmacology: effects of drugs on glucose metabolism

Range:
0.02-10U/L glucose oxidase for colorimetric assays and 0.002-1.5U/L for fluorometric assays.

Detection limit:
0.02 (OD), 0.002U/L (FL)

Kit Components:
Assay Buffer, 1x10ml
Glucose, 1x1.5ml 2M Glucose
Standard, 1x100ul 3% H2O2
HRP Enzyme, 1x120ul
Dye Reagent. 1x120ul

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.