Anpep Rat Monoclonal Antibody [Clone ID: 123H1]
Specifications
Product Data | |
Clone Name | 123H1 |
Applications | FC, IP |
Recommended Dilution | Immunoprecipitation: 5 μg/1000 μl of cell extract from 5x106 cells. Flow Cytometry: 5-10 μg/ml (final concentration). For details see Protocol below. |
Reactivities | Mouse |
Host | Rat |
Isotype | IgG2b |
Clonality | Monoclonal |
Immunogen | Murine DC from C57BL/6 mice |
Specificity | This antibody recognizes Mouse CD13. Other species not tested. |
Formulation | PBS containing 50% Glycerol, pH 7.2. No preservative is contained. State: Azide Free State: Liquid Ig fraction |
Concentration | lot specific |
Purification | Protein G Agarose Chromatography |
Conjugation | Unconjugated |
Storage | Upon receipt, store (in aliqouts) at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
Gene Name | alanyl (membrane) aminopeptidase |
Database Link | |
Background | CD13 is a myeloid differentiation molecule expressed on committed myeloid progenitors, granulocytes, monocytes, and leukemic cells of myeloid origin. It is also expressed on nonhematopoietic cells including fibroblasts, renal proximal tubule, and small intestine brush-border membrane. The gene for CD13 has recently been cloned the cDNA sequence shows that CD13 is the metalloprotease aminopeptidase N. Biochemical studies have shown that CD13 is a 150-kDa glycoprotein, which exists as a 130-kDa intracellular precursor form. This 130-kDa precursor molecule is post-translationally modified in the Golgi apparatus to produce the 150-kDa mature cell surface form of the molecule. |
Synonyms | Aminopeptidase N, ANPEP, APN, PEPN |
Note | This product was originally produced by MBL International. Protocol: Flow Cytometric analysis for floating cells Protocol 2 Immunoprecipitation |
Reference Data |
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