Formaat : 5mg
Synonyms: 19-ethynyl Cholesterol
| Description | 19-alkyne Cholesterol is a bioorthogonal cholesterol analog used to investigate sterol localization, metabolism, and cholesterol-associated Hedgehog (Hh) signal transduction. 19-alkyne Cholesterol incorporates into cellular membranes and can be detected by copper(I)-catalyzed azide-alkyne cycloaddition with fluorescent reporter azides, enabling high-resolution visualization of cholesterol distribution in NIH3T3 cells and subcellular compartments. 19-alkyne Cholesterol also supports research into cholesterol-dependent Hh pathway biology in Shh-LIGHT2 cells, providing a research tool for studying sterol trafficking, membrane organization, and signaling. |
| Synonyms | 19-ethynyl Cholesterol |
| Molecular Weight | 396.7 |
| Formula | C28H44O |
| Cas No. | 2058319-71-2 |
| Smiles | C(#C)[C@@]12[C@@]3([C@]([C@]4([C@](C)(CC3)[C@@]([C@@H](CCCC(C)C)C)(CC4)00)00)(CC=C1C[C@@H](O)CC2)00)00 |
| Storage | Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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