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4-Hydroxynonenal [75899-68-2]

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Referentie NB-64-01309-5mg

Formaat : 5mg

Merk : Neo Biotech

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4-Hydroxynonenal

(Synonyms: 4-HNE) Copy Product Info

Synonyms: 4-HNE

Catalog No. T10148 Copy Product Info

Purity: 99.50%

4-Hydroxynonenal (4-HNE) (4-HNE) is an oxidative/nitrosative stress biomarker. It is a substrate and an inhibitor of acetaldehyde dehydrogenase 2 (ALDH2).

Cas No. 75899-68-2

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.50%

Color:Yellow

Product Introduction

Bioactivity

Description	4-Hydroxynonenal (4-HNE) (4-HNE) is an oxidative/nitrosative stress biomarker. It is a substrate and an inhibitor of acetaldehyde dehydrogenase 2 (ALDH2).
In vitro	4-Hydroxynonenal (4-HNE) is both a substrate and an inhibitor of ALDH2, with inhibition reversible at low concentrations and becoming irreversible at 10 μM. It induces antioxidant defense mechanisms to limit its own production and enhance cellular protection against oxidative stress. As a product of lipid peroxidation, 4-HNE is mutagenic and genotoxic in viruses, bacteria, and mammalian cells, reacting with all four DNA bases with varying efficiency (G > C > A > T). 4-Hydroxynonenal-dG serves as the best biomarker of its genotoxic effects, primarily found in nuclear DNA [1].
In vivo	Twenty-four hours after fluid percussion injury (FPI), mouse brain tissue is analyzed for the expression levels of NADPH oxidase 1 (NOX1), inducible nitric oxide synthase (iNOS), and 4-Hydroxynonenal (4-HNE). Both wild-type (Nrf2+/+) and Nrf2-deficient (Nrf2-/-) mice show increased 4-HNE expression following a moderate 15 psi injury compared to uninjured controls. In Nrf2-/-knockout mice, 4-HNE expression is significantly higher than in corresponding injured and uninjured Nrf2-/-wild-type animals [2].
Synonyms	4-HNE

Chemical Properties

Molecular Weight	156.22
Formula	C₉H₁₆O₂
Cas No.	75899-68-2
Smiles	CCCCCC(O)\C=C\C=O
Relative Density.	0.941 g/cm3

Storage & Solubility Information

Storage

Store at low temperature Pure form: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 249 mg/mL (1593.91 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (32.01 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	6.4012 mL	32.0061 mL	64.0123 mL	320.0615 mL
5 mM	1.2802 mL	6.4012 mL	12.8025 mL	64.0123 mL
10 mM	0.6401 mL	3.2006 mL	6.4012 mL	32.0061 mL
20 mM	0.3201 mL	1.6003 mL	3.2006 mL	16.0031 mL
50 mM	0.1280 mL	0.6401 mL	1.2802 mL	6.4012 mL
100 mM	0.0640 mL	0.3201 mL	0.6401 mL	3.2006 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

stressoxidativenitrosativemitochondriaInhibitorinhibitHuman Endogenous MetabolitegenotoxicEndogenousMetaboliteEndogenous MetaboliteCancerbiomarkerALDH2ALDHAldehyde Dehydrogenase (ALDH)4-Hydroxynonenal4Hydroxynonenal4 Hydroxynonenal

Related Tags: 4-Hydroxynonenal chemical structure | 4-Hydroxynonenal in vivo | 4-Hydroxynonenal in vitro | 4-Hydroxynonenal formula | 4-Hydroxynonenal molecular weight