Disease:

Malignant Melanoma

Origin:

Derived from a 54 year old female with malignant melanoma. The cell line produces rapidly growing amelanotic melanomas in anti-thymocyte serum treated NIH Swiss mice. A375 cell line has been used to obtain paracrine factors for prolonged culture of mesenchymal stromal cells (MSCs). It has also been used to study oncolytic activity of the peptide LTX-315.

Species:

Homo sapiens, human

Tissue:

Skin

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

It is a hypotriploid with a modal number of 62 chromosomes. There are 9 marker chromosomes that are commonly found in each cell, and normal N2, N6, and N22 are present at one copy per cell.

Patient:

Female, 54 years of age

Medium:

AddexBio-formulated DMEM (C0003-01) + 10% FBS

Subculture:

Subculture at a ratio of 1:3 to 1:8 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.

Freezing Medium:

Complete growth culture medium supplemented with 5% (v/v) DMSO