A8011-1g | Biotin-tyramide [41994-02-9] Clinisciences

Biotin-tyramide is used for tyramide signal amplification (TSA) which is a powerful, patented technology that significantly enhances both chromogenic and fluorescent signals. TSA is easily integrated into standard nonradioactive in situ hybridization (ISH) or IHC protocols.

TSA is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to catalyze the deposition of a fluorophore-labeled tyramide amplification reagent onto tissue sections or cell preparation surfaces that have been previously blocked with proteins.

Biotin-tyramide use horseradish peroxidase (HRP) to catalyze covalent deposition of biotin labels directly adjacent to the immobilized enzyme. The labeling reaction is quick (less than 10 minutes) and deposited labels can be detected with streptavidin conjugates for imaging in brightfield or fluorescence microscopy.

1. Colten Mitchell McEwan. "The Discovery of Novel 14-3-3 Binding Proteins ATG9A and PTOV1 and Their Role in Regulating Cancer Mechanisms." Brigham Young University. 2022.
2. Yao Fan, Yi Liang, et al. "PRKDC promotes hepatitis B virus transcription through enhancing the binding of RNA Pol II to cccDNA." Cell Death Dis. 2022 Apr 25;13(4):404. PMID: 35468873
5. Wei Qin, Samuel A. Myers, et al. "Spatiotemporally-resolved mapping of RNA binding proteins via functional proximity labeling reveals a mitochondrial mRNA anchor promoting stress recovery." Nat Commun. 2021 Aug 17;12(1):4980. PMID: 34404792
3. Eugene Joeh, Abigail E Reeves, et al. "Mapping Interactions between Glycans and Glycan‐Binding Proteins by Live Cell Proximity Tagging." Curr Protoc. 2021 Apr;1(4):e104. PMID: 33861898
4. Chen Zong, Fan Jiang, et al. "Imaging sensor array coupled with dual-signal amplification strategy for ultrasensitive chemiluminescence immunoassay of multiple mycotoxins." Biosens Bioelectron. 2021 Apr 1;177:112998. PMID: 33486134
6. Clemens AM, Lenschow C, et al. "Estrus-Cycle Regulation of Cortical Inhibition." Curr Biol. 2019 Feb 18;29(4):605-615.e6. PMID: 30744972
7. Liu P, Huang J, et al. "Mammalian mitochondrial RNAs are degraded in the mitochondrial intermembrane space by RNASET2." Protein Cell. 2017 Oct;8(10):735-749. PMID: 28730546

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Physical Appearance	A solid
Storage	Store at -20°C
M.Wt	363.47
Cas No.	41994-02-9
Formula	C₁₈H₂₅N₃O₃S
Synonyms	Biotin-Phenol,Biotin Phenol
Solubility	insoluble in H2O; ≥100.2 mg/mL in DMSO; ≥8.18 mg/mL in EtOH with ultrasonic
Chemical Name	N-(4-hydroxyphenethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide
SDF	Download SDF
Canonical SMILES	O=C1N[C@@](14)([C@](14)(CS2)N1)[C@@H]2CCCCC(NCCC3=CC=C(O)C=C3)=O
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.