TP750180-B | Biotinylated recombinant SARS coronavirus 2 Nucleocapsid

Biotinylated recombinant SARS coronavirus 2 Nucleocapsid Protein (N-protein)

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TP750180-B

Purified Biotinylated recombinant SARS coronavirus 2 Nucleocapsid Protein (N-protein) with N-terminal HIS tag and site-specific Biotinylation on the C-terminal AVIplus tag

Purified recombinant protein N protein was analyzed by SDS-PAGE gel and Coomossie Blue Staining.

Specifications
Product Data
Species	SARS-CoV-2
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence MSDNGPQNQR NAPRITFGGP SDSTGSNQNG ERSGARSKQR RPQGLPNNTA SWFTALTQHG KEDLKFPRGQ GVPINTNSSP DDQIGYYRRA TRRIRGGDGK MKDLSPRWYF YYLGTGPEAG LPYGANKDGI IWVATEGALN TPKDHIGTRN PANNAAIVLQ LPQGTTLPKG FYAEGSRGGS QASSRSSSRS RNSSRNSTPG SSRGTSPARM AGNGGDAALA LLLLDRLNQL ESKMSGKGQQ QQGQTVTKKS AAEASKKPRQ KRTATKAYNV TQAFGRRGPE QTQGNFGDQE LIRQGTDYKH WPQIAQFAPS ASAFFGMSRI GMEVTPSGTW LTYTGAIKLD DKDPNFKDQV ILLNKHIDAY KTFPPTEPKK DKKKKADETQ ALPQRQKKQQ TVTLLPAADL DDFSKQLQQS MSSADSTQA
Tag	N-HIS and C-AVIPLUS
Predicted MW	49.8 kDa
Concentration	>0.05 µg/µL as determined by Bradford protein assay method.
Purity	> 90% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, pH 8.0, 250 mM NaCl, 10% glycerol
Bioactivity	The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping	Dry Ice
Reference Data
RefSeq	QHD43423

FAQs
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SDS
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Recombinant Protein Resources
Video tutorial: Why protein expression host matters Webinar Video: Overexpression lysate as positive controls for Western blot Product Overview Video: Overexpression lysates as positive controls for Western blot Video Tutorial: Knockout(KO) lysate and antibody specificity Product Brochure: Protein

Citations

COVID-19 Lung Pathogenesis in SARS-CoV-2 Autopsy Cases
Valdebenito, Bessis, Annane et al
Front Immunol (2021) 12, 735922 DOI: 10.3389/fimmu.2021.735922

Cited by 66

AI Summary: OriGene contributed to this study by providing the antibody for SARS protein M (APO90991su-n), which was used to detect viral markers in lung tissue samples from COVID-19 autopsy cases.

Snippet: Vimentin (sc-52721) from Santa Cruz (Santa Cruz, CA) and the antibody for SARS protein M (APO90991su-n) were obtained from Origene (Rockville, MD)

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Biotinylated recombinant SARS coronavirus 2 Nucleocapsid Protein (N-protein)

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Biotinylated recombinant SARS coronavirus 2 Nucleocapsid Protein (N-protein)

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