Reagentia en instrumenten voor immunologie, celbiologie en moleculaire biologie.

BIS-TRIS propane [64431-96-5]

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Referentie NB-64-35207-1mL

Formaat : 1mLx10mM(inDMSO)

Merk : Neo Biotech

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BIS-TRIS propane

(Synonyms: BTP) Copy Product Info

Synonyms: BTP

Catalog No. T65636 Copy Product Info

Purity: 99.19%

BIS-TRIS propane (BTP), a novel multidentate ligand of nickel- and cobalt-based spin clusters, is a metal chelator. BIS-TRIS propane is often used as a zwitterionic buffer for PCR and protein crystallization studies.

Cas No. 64431-96-5

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.19%

Appearance:Solid

Color:White

Product Information

Bioactivity

Description	BIS-TRIS propane (BTP), a novel multidentate ligand of nickel- and cobalt-based spin clusters, is a metal chelator. BIS-TRIS propane is often used as a zwitterionic buffer for PCR and protein crystallization studies.
Synonyms	BTP

Chemical Properties

Molecular Weight	282.33
Formula	C₁₁H₂₆N₂O₆
Cas No.	64431-96-5
Smiles	OCC(CO)(CO)NCCCNC(CO)(CO)CO

Storage & Solubility Information

Storage

Keep away from direct sunlight, Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 22.73 mg/mL (80.51 mM), Sonication is recommended.

H2O: 80 mg/mL (283.36 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 1 mg/mL (3.54 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/H2O

	1mg	5mg	10mg	50mg
1 mM	3.5420 mL	17.7098 mL	35.4195 mL	177.0977 mL
5 mM	0.7084 mL	3.5420 mL	7.0839 mL	35.4195 mL
10 mM	0.3542 mL	1.7710 mL	3.5420 mL	17.7098 mL
20 mM	0.1771 mL	0.8855 mL	1.7710 mL	8.8549 mL
50 mM	0.0708 mL	0.3542 mL	0.7084 mL	3.5420 mL

H2O

	1mg	5mg	10mg	50mg
100 mM	0.0354 mL	0.1771 mL	0.3542 mL	1.7710 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Related Tags: BIS-TRIS propane chemical structure | BIS-TRIS propane formula | BIS-TRIS propane molecular weight