CCK ELISA Kit (Human)
Referentie OKEH00596
Formaat : 96Wells
Merk : Aviva Systems Biology
CCK ELISA Kit (Human) : 96 Wells (OKEH00596)
| Datasheets/Manuals | Printable datasheet for CCK ELISA Kit (Human) : 96 Wells (OKEH00596) |
|---|---|
| COA Datasheet | Printable COA datasheet for OKEH00596 |
| Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology CCK ELISA Kit (Human) (OKEH00596) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human CCK antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CCK and incubated. The CCK found in the sample or standards competes with the biotinylated CCK for limited binding sites on the immobilized anti-human CCK antibody. Excess unbound biotinylated CCK and sample or standard CCK is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CCK captured in the well and inversely proportional to the amount of CCK which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 15.6-1000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.5% (n = 20) Mean Inter-assay CV%: < 7.6% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
| ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 10 pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Human Cholecystokinin | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA |
| Gene Symbol | CCK |
|---|---|
| Gene Full Name | cholecystokinin |
| Alias Symbols | cholecystokinin, cholecystokinin triacontatriapeptide, prepro-cholecystokinin. |
| NCBI Gene Id | 885 |
| Protein Name | Cholecystokinin |
| Description of Target | This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion. |
| Uniprot ID | P06307 |
| Protein Accession # | NP_000720.1 |
| Nucleotide Accession # | NM_000729.5 |
| Protein Size (# AA) | 115 |
| Molecular Weight | 12 kDa |