Off White, homogeneous, free flowing powder

Cell Culture Grade

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Light yellow, clear, complete

5.7

Dissolve 17.03g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 1.2g sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

<1 EU/ml

Amino Acids:
L-Alanine 8.9
L-Arginine 52.26
L-Asparagine 132.1
L-Aspartic Acid 13.31
L-Cysteine 24.44
L-Glutamic Acid 14.71
L-Glutamine 146.0
Glycine 7.51
L-Histidine 15.52
L-Isoleucine 13.12
L-Leucine 39.36
L-Lysine 29.24
L-Methionine 4.48
L-Phenylalanine 4.96
L-Proline 5.76
L-Serine 31.53
L-Threonine 35.73
L-Tryptophan 6.13
L-Tyrosine 9.06
L-Valine 35.16

Inorganic Salts:
Ammonium Metavandate: 0.000585
Ammonium Molybdate: 0.0012359
Calcium Chloride-2H20: 22.0
Cupric Sulfate -5H20: 0.0002497
Ferric Nitrate-9H20: 1.39
Magnesium Sulfate: 180.57
Manganese Sulfate-5H2O: 0.0001205
Nickel Chloride Hexahydrate: 0.0000011885
Potassium Chloride: 186.25
Potassium Phosphate Mono: 68.05
Sodium Chloride: 7183.2
Sodium Metasilicate-9H2O: 0.1421
Sodium Phosphate Dibasic: 71.05
Sodium Selenite: 0.00789
Stannous Chloride Dihydrate: 0.000001128
Zinc Sulfate-7H2O: 0.02875

Vitamins:
Biotin: 0.00733
Choline Chloride 13.96
Vitamin B12 0.1355
Folinic Acid, Calcium: 0.0006016
Myo-Inositol: 18.02
Nicotinamide: 6.1
D-Pantothenic Acid-Ca: 0.2383
Pyridoxine-HCl: 0.0616
Riboflavin: 0.1129
Thiamine-HCl: 0.337

Other:
Adenine: 0.1350
Thymidine: 0.07266
D-Glucose: 1440.0
HEPES Free Acid: 7149.0
Linoleic Acid: 0.0561
Lipoic Acid: 0.00206
Phenol Red, Sodium: 1.242
Putrescine-2HCl: 0.0001611
Sodium Pyruvate: 55.0

Total: 17.03 g/liter

Culture Media; Media-Cell Culture; MCDB