CultureBoost-R™ contains Recombinant Human Growth Factors.

This 10mL vial supplements 500mL Cell Systems Medium.

CultureBoost-R™ is the recombinant growth factor supplement for Cell Systems Complete Classic Medium (cat. #4Z0-500-R) and Cell Systems Medium Minus Serum (cat. #4Z3-500-R). CultureBoost-R™ contains defined growth factors and porcine heparin.

CultureBoost-R™ is produced by bacterial fermentation of E. coli. The product's working concentration is 10ng/mL. Biological activity is adjusted to 110% of maximum using CSC 2V0 (Human Venous Endothelial) cells, ACBRI 181 (Primary Retinal Microvascular Endothelial) cells, and ACBRI 376 (Primary Brain Microvascular Endothelial) cells.

CultureBoost-R™ is qualified for use with all Cell Systems media.

Available Formats

This product is included as a 10mL vial in various Cell Systems medium kits and in "The System™". Each 10ml vial supplements 500mL medium. Individual 10mL vials are also available separately.

Use

CultureBoost-R™ has two main uses.

1. Application as a growth supplement to medium:

Cell Systems Complete Medium Kit (cat. #4Z0-500-R) and Cell Systems Medium Minus Serum Kit (cat. #4Z3-500-R) each include a 10mL vial of CultureBoost-R™ (cat. #4CB-500-R).

CultureBoost-R™ can be purchased separately (cat. #4CB-500-R) to supplement Cell Systems Media Kit without Growth Factor (cat. # 4Z0-500-S) or other cell culture media.

2. Application in the vial thaw procedure:

Cells are at their most fragile when in recovery from thawing or passage. The growth factors in CultureBoost™ and CultureBoost-R™ stabilize the plasma membrane and provide a concentrated dose of factors that have been shown to aid the cell in attachment, activation, and achievement of polarity.

CultureBoost-R™ can be beneficially used any time cells are "loosened" from the pellet prior to resuspension in Cell Systems Medium in preparation for plating. The best results of using CultureBoost-R™ are evident when 50-100 microliters are added to the loosened cell pellet just prior to re-suspension in medium. Please refer to the instructions for our Passage Reagent Group™ (cat. #4Z0-800).

Handling and Storage

This product is delivered at ambient temperature. Use 10ml to supplement 500ml cell culture medium. If the entire unit will not be used within 30 days, aliquot and freeze. Once opened, shelf life is 30 days at +2-8°C.

CultureBoost-R™

Referentie 4CB-500-R

Merk : Cell Systems Corporation

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