D-Lactic Acid/Lactate(LA)Colorimetric Assay Kit

Referentie E-BC-K002-M-96T

Formaat : 96T

Merk : Elabscience

Neem contact op met een lokale distributeur :


Telefoonnummer : +1 850 650 7790

Detection Principle Using NAD+ as H+ receptor, D-lactate dehydrogenase (LDH) catalyzes the reaction of D-lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of D-lactic acid can be calculated.
Synonyms D-LA
Sample Type Serum,Plasma,Animal tissue,Saliva sample
Detection Method Colorimetric method
Detection Instrument Microplate reader (520-550 nm, optimum wavelength: 530 nm)
Research Area Glycolysis And Lipid Metabolism
Other Reagents Required PBS (0.01 mol/L, pH 7.4)
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.06 mmol/L
Detection Range 0.06-8.0 mmol/L
Precision inter-assay CV: 7.7% | intra-assay CV: 3.8%
Assay Time 20 min
Cat.No. Product Name Sizes
E-BC-K044-M L-Lactic Acid (LA) Colorimetric Assay Kit 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human plasma 1
Human serum 1
Rat serum 1
Rat plasma 1
Mouse serum 1
Rabbit serum 1
10% Rat kidney tissue homogenate 2-3
10% Mouse brain tissue homogenate 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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E-AB-31662-60
Discontinued
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E-BC-K044-S-100
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