DAPI, 10mg/mL in H2O

Referentie 40043

Formaat : 1ML

Merk : Biotium

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DAPI

DAPI is a popular blue fluorescent DNA dye. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement.

Technology

Cellular Stains
Considerations for Immunofluorescence Staining

Tech Tips

Hoechst & DAPI Staining Protocols – Cell Staining with Hoechst or DAPI Nuclear Stains
Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst
Tech Tip: Battling Tissue Autofluorescence
Troubleshooting Tips for Fluorescence Staining

Product Description

DAPI (4′,6-Diamidino-2-Phenylindole, dihydrochloride) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining, and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions.

  • Classic blue nuclear counterstain for fixed cells
  • Available as a powder or solution
  • λExEm (with DNA) = 358/461 nm
  • Store at 4°C and protect from light, especially in solution

DAPI as a dilactate powder is more water soluble than the dihydrochloride salt, and therefore a better choice for preparing stock solutions in water. If mounting media will be used, we also offer anti-fade mounting media containing DAPI, in both hardset and wetset formulations. Having trouble with your experiments? See our section on troubleshooting tips for fluorescent staining.

Product Catalog Number MW Unit Size Format
DAPI Powder
(dilactate)
40009 457.49 10 mg Yellow solid
DAPI Powder
(dihydrochloride)
40011 350.25 10 mg Yellow solid
DAPI Solution
(dilactate)
40043 457.49 1 mL
(10 mg/mL in water)
Yellow solution

Find the Right Nuclear Stain for Your Application

DAPI can be used to stain mammalian cells as well as gram-positive and gram-negative bacteria. In yeast, the staining is weak and not nuclear. DAPI is less membrane permeant than Hoechst, and is typically used to stain fixed cells. On the other hand, Hoechst dyes are membrane-permeant and more often used for live or fixed cell staining and cell cycle analysis. While Hoechst and DAPI show less cytotoxicity than intercalating DNA dyes, they bind DNA in living cells and are potentially hazardous. DAPI and Hoechst undergo photoconversion by UV excitation to form green fluorescent dyes, which can lead to artifacts in multi-color imaging. See our Tech Tip Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst for more information.

Biotium also offers unique NucSpot® Nuclear Stains for bright and specific nuclear staining in dead or fixed cells. The stains are available in a wide range of colors from green to near-IR. See our Cellular Stains Selection Guide and Cellular Stains Table for more information on other nuclear stains we offer.

DAPI, dilactate
DAPI, dilactate
DAPI, hydrochloride
DAPI, hydrochloride

References

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  2. Front. Cell Dev. Biol., 7, 321, (2020), DOI: 10/ggt677
  3. Int. J. Biol. Sci., 16, 1586, (2020), DOI: 10/ggt7nv
  4. Poultry Science, 99, 1209, (2020), DOI: 10/ggt7cj
  5. Acta Biomaterialia, 107, 138, (2020), DOI: 10/ggt8d9
  6. Int. J. Med. Sci., 17, 480, (2020), DOI: 10/ggt8fb
  7. Nat Commun, 11, 1416, (2020), DOI: 10/ggt74m
  8. Polymers, 12, 329, (2020), DOI: 10/ggt8fg
  9. Biomed Pharmacother., 119, 109420, (2019), DOI: 10/ggt7cw
  10. Redox Biology, 26, 101220, (2019), DOI: 10/ggt7dg
  11. Front. Cell Dev. Biol., 7, 224, (2019), DOI: 10/ggszm9
  12. Sci Rep, 9, 2102, (2019), DOI: 10/ggt7mq
  13. Nanoscale, 11, 18255, (2019), DOI: 10/ggt8ff
  14. Am J Physiol Gastrointest Liver Physiol, 317, G531, (2019), DOI: 10/ggt8fh
  15. Ecotoxicol Environ Saf., 183, 109583, (2019), DOI: 10/ggt7c9
  16. Biotechnic Histochem, 70, 220, (1995), DOI: 10.3109/10520299509108199
 

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