Orb623166-1l | General Block ELISA Blocking Buffer Clinisciences

General Block ELISA Blocking Buffer

SKU: orb623166

Description

Contains a mammalian protein blocking agent suitable for most antibody capture ELISA formats and peptide/protein antigen-down ELISA formats. It provides a stable long-term environment for dried antigen or antibody and minimizes non-specific binding.

Images & Validation

−

Application Notes	1. Coat antibody or antigen onto the ELISA plate using ICT’s Antibody Coating Buffer or Antigen Coating Buffer. 2. Incubate 8–24 hours at room temperature (RT). 3. Aspirate the coating solution. 4. Wash each well twice with ICT’s ELISA Wash Buffer. 5. Block the uncoated regions of the ELISA plate by pipetting 300–400 µL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution. 6. Incubate 8–24 hours at RT. For best blocking, incubate overnight at RT. 7. Aspirate the blocking buffer; do not wash. 8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8°C.

Application Notes

1. Coat antibody or antigen onto the ELISA plate using ICT’s Antibody Coating Buffer or Antigen Coating Buffer. 2. Incubate 8–24 hours at room temperature (RT). 3. Aspirate the coating solution. 4. Wash each well twice with ICT’s ELISA Wash Buffer. 5. Block the uncoated regions of the ELISA plate by pipetting 300–400 µL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution. 6. Incubate 8–24 hours at RT. For best blocking, incubate overnight at RT. 7. Aspirate the blocking buffer; do not wash. 8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8°C.

Key Properties

−

Storage & Handling

−

Storage	2-8°C
Concentration	1 X
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

−

General Block ELISA Blocking Buffer [orb1821224]
Sizes Available: 500 ml
Block Buffer Optimization Pack [orb1409891]
Sizes Available: 5 x 100 ml
General Block ELISA Blocking Buffer [orb1821225]
Sizes Available: 100 ml

General Block ELISA Blocking Buffer

Referentie orb623166-1l

Merk : Biorbyt

General Block ELISA Blocking Buffer

Description

Images & Validation

Key Properties

Storage & Handling

General Block ELISA Blocking Buffer [orb1821224]

Block Buffer Optimization Pack [orb1409891]

General Block ELISA Blocking Buffer [orb1821225]

Protocol Information