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> HCN1 Antibody

HCN1 Antibody

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Referentie ASM10182-A565-100

Formaat : Onrequest

Merk : Abcepta

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  • IHC - HCN1 Antibody ASM10182
    Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (ASM10182). Tissue: Cerebellum. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (ASM10182) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Cytoplasmic staining of Purkinje cells.
  • WB - HCN1 Antibody ASM10182
    Western Blot analysis of Rat brain membrane lysate showing detection of HCN1 protein using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (ASM10182). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (ASM10182) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
  • IHC - HCN1 Antibody ASM10182
    Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (ASM10182). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (ASM10182) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
  • IHC - HCN1 Antibody ASM10182
    Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (ASM10182). Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (ASM10182) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Neurons.
  • IHC - HCN1 Antibody ASM10182
    Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (ASM10182). Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (ASM10182) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP
Primary Accession Q9JKBO
Other Accession NP_445827.1
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat HCN1 Monoclonal IgG1
Target/Specificity Detects ~100kDa. No cross-reactivity against HCN2.
Other Names BCNG-1 Antibody, BCNG1 Antibody, Brain cyclic nucleotide gated channel 1 Antibody, Brain cyclic nucleotide-gated channel 1 Antibody, HAC2 Antibody, HCN1 Antibody, HCN1_HUMAN Antibody, Hyperpolarization activated cyclic nucleotide gated potassium channel 1 Antibody, Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 1 Antibody, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 Antibody
Clone Names S70-28
Immunogen Fusion protein amino acids 778-910 (C terminus) of rat HCN1
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-304 was sufficient for detection of HCN1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Membrane