K2122-100 assays | HDL and LDL/VLDL Quantification Colorimetric/Fluorometric

Background

High-density lipoprotein (HDL), low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL) are three of the five major groups of lipoproteins and transport lipids within the water around cells. High levels of LDL and low levels of HDL are related to an increased risk of cardiovascular diseases. Regulation of LDL and HDL play critical roles in various disease developments.

The HDL and LDL/VLDL Quantification Colorimetric/Fluorometric Kit provides a sensitive, fast and convenient way for detection of HDL and LDL/VLDL in plasma and serum samples based on a convenient separation of HDL from LDL and VLDL, and colorimetric and fluorometric method. In the assay, cholesterol oxidase specifically recognizes free cholesterol and generates products which react with probe to yield fluorescence (Ex/Em = 538/587 nm) and color (570 nm). Cholesteryl ester can be hydrolized into free cholesterol by cholesterol esterase, therefore, cholesterol ester and free cholesterol can be separately detected in the absence and presence of cholesterol esterase in the reactions.

Features & Properties

Features	Fast and convenient; Simple procedure; Takes approx. 1 hour.
Shipping	Gel pack.
Storage Conditions	Store at -20°C.

Quality Control

DataSheet

Storage

Store at -20°C.

Overview

Kit components

Cholesterol Assay Buffer, 2X LDL/VLDL Precipitation Buffer, Cholesterol Probe (in DMSO, anhydrous), Enzyme Mix (Lyophilized), Cholesterol Esterase (Lyophilized), Cholesterol Standard (2 µg/µL)

HDL and LDL/VLDL Quantification Colorimetric/Fluorometric Kit

Referentie K2122-100 assays

Neem contact op met een lokale distributeur :