Orb1736780-48Tests | Human Breast Cancer Type 1 Susceptibility Protein

Human Breast Cancer Type 1 Susceptibility Protein (BRCA1) ELISA Kit

SKU: orb1736780

Description

The Human Breast Cancer Type 1 Susceptibility Protein (BRCA1) ELISA Kit uses a sandwich enzyme immunoassay to quantify Breast Cancer Type 1 Susceptibility Protein (BRCA1) in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 0.32-20 ng/mL and a sensitivity of 0.15 ng/mL.

Research Area

Cancer Biology

Images & Validation

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Application Notes	standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BRCA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BRCA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BRCA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BRCA1 in the samples is then determined by comparing the OD of the samples to the standard curve

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BRCA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BRCA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BRCA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BRCA1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.15 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53

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Human BRCA1 ELISA Kit [orb562461]
Reactivity:
Human
Dynamic Range:
0.312-20ng/ml
Sensitivity:
0.188ng/ml
Sizes Available: 96 T, 48 T
Human BRCA1 (Breast cancer type 1 susceptibility protein) ELISA Kit [orb2648116]
Reactivity:
Human
Dynamic Range:
0.313-20ng/ml
Sensitivity:
0.188ng/ml
Sizes Available: 48 T, 96 T

Human Breast Cancer Type 1 Susceptibility Protein (BRCA1) ELISA Kit

Referentie orb1736780-48Tests

Merk : Biorbyt

Human Breast Cancer Type 1 Susceptibility Protein (BRCA1) ELISA Kit

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

Human BRCA1 ELISA Kit [orb562461]

Human BRCA1 (Breast cancer type 1 susceptibility protein) ELISA Kit [orb2648116]

Protocol Information

Reactivity:	Human
Dynamic Range:	0.312-20ng/ml
Sensitivity:	0.188ng/ml