RayBio® C-Series Human Cytokine Antibody Array 6 Kit. Detects 60 Human Cytokines. Suitable for all liquid sample types.
Human Cytokine Array C6
Human Cytokine Array C6
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 60 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Shipping Type | Blue ice |
| Storage | -20°C |
| Storage / Stability |
|
| More Information | Suggested Application Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Angiogenin
| BDNF
| BLC (CXCL13)
| BMP-4
| BMP-6
|
Ck beta 8-1 (CCL23)
| CNTF
| EGF
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
|
Eotaxin-3 (CCL26)
| FGF-6
| FGF-7 (KGF)
| Flt-3 Ligand
| Fractalkine (CX3CL1)
|
GCP-2 (CXCL6)
| GDNF
| GM-CSF
| I-309 (TCA-3/CCL1)
| IFN-gamma
|
IGFBP-1
| IGFBP-2
| IGFBP-4
| IGF-1
| IL-10
|
IL-13
| IL-15
| IL-16
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
|
IL-1 ra (IL-1 F3)
| IL-2
| IL-3
| IL-4
| IL-5
|
IL-6
| IL-7
| Leptin
| Light (TNFSF14)
| MCP-1 (CCL2)
|
MCP-2 (CCL8)
| MCP-3 (MARC/CCL7)
| MCP-4 (CCL13)
| M-CSF
| MDC (CCL22)
|
MIG (CXCL9)
| MIP-1 delta (CCL15)
| MIP-3 alpha (CCL20)
| NAP-2 (PPBP/CXCL7)
| NT-3
|
PARC (CCL18)
| PDGF-BB
| RANTES (CCL5)
| SCF
| SDF-1 alpha (CXCL12 alpha)
|
TARC (CCL17)
| TGF beta 1
| TGF beta 3
| TNF alpha
| TNF beta (TNFSF1B)
|
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array C6 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Specific Features of Stromal Cells Isolated from the Two Layers of Subcutaneous Adipose Tissue: Roles of Their Secretion on Angiogenesis and Neurogenesis
- Species:
- Human
- Sample Type:
- Human-adipose-tissue-derived mesenchymal stromal cells (AD-MSCs)
- Year:
- 2023
- Journal Name:
- Journal of clinical medicine
CD146+ Endometrial-Derived Mesenchymal Stem/Stromal Cell Subpopulation Possesses Exosomal Secretomes with Strong Immunomodulatory miRNA Attributes
- Species:
- Human
- Sample Type:
- THP-1 cell, human peripheral blood mononuclear cells (PBMC)
- Year:
- 2022
- Journal Name:
- Cells
Anti-Inflammatory Effect of Specialized Proresolving Lipid Mediators on Mesenchymal Stem Cells: An In Vitro Study
- Species:
- Human
- Sample Type:
- human bone marrow-derived mesenchymal stem cells (hBMMSCs)
- Year:
- 2022
- Journal Name:
- Cells
Vasopressin Receptor Type-2 Mediated Signaling in Renal Cell Carcinoma Stimulates Stromal Fibroblast Activation
- Species:
- Human
- Sample Type:
- Caki1 cell
- Year:
- 2022
- Journal Name:
- International Journal of Molecular Sciences
Conditioned medium of amniotic fluid-derived stromal cells exerts a bone anabolic effect by enhancing progenitor population and angiogenesis
- Species:
- Human
- Sample Type:
- amniotic fluid-derived stromal cells(AFSC-CM)
- Year:
- 2022
- Journal Name:
- Journal of Tissue Engineering and Regenerative Medicine
The Secretome of Aged Fibroblasts Promotes EMT-Like Phenotype in Primary Keratinocytes from Elderly Donors through BDNF-TrkB Axis
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2020
- Journal Name:
- Journal of Investigative Dermatology
Soluble Compounds Released by Hypoxic Stroma Confer Invasive Properties to Pancreatic Ductal Adenocarcinoma
- Species:
- Human
- Sample Type:
- Conditioned Media (HPS, Hypoxic PS1 supernates)
- Year:
- 2020
- Journal Name:
- Biomedicines
Plasma Macrophage Migration Inhibitory Factor and CCL3 as Potential Biomarkers for Distinguishing Patients with Nasopharyngeal Carcinoma from High-Risk Individuals Who Have Positive Epstein-Barr Virus Capsid Antigen-Specific IgA
- Species:
- Human
- Sample Type:
- Plasma (Nasopharyngeal carcinom patients biomarker)
- Year:
- 2019
- Journal Name:
- Cancer research and treatment
- PubMed ID:
- 29807404
A novel model of humanised keloid scarring in mice
- Species:
- Human
- Sample Type:
- Conditioned Media (Fibroblast cell lines in keloid scarring model)
- Year:
- 2018
- Journal Name:
- International Wound Journal
- PubMed ID:
- 29076632
HIV-Nef and ADAM17-Containing Plasma Extracellular Vesicles Induce and Correlate with Immune Pathogenesis in Chronic HIV Infection
- Species:
- Human
- Sample Type:
- Extracellular Vesicles (plasma Extracellular vesicles of HIV-NEF and ADAM17 chronic HIV patients)
- Year:
- 2016
- Journal Name:
- EBioMedicine