RayBio® G-Series Human Obesity Antibody Array 1 Kit. Detects 62 Human Adipokines. Suitable for all liquid sample types.
Human Obesity Array G1
Human Obesity Array G1
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Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 62 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Cardiovascular Disease, Energy Metabolism, Obesity & Metabolic Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| 4-1BB (TNFRSF9/CD137)
| ACE-2
| Adiponectin (ACRP30)
| Adipsin (Complement Factor D)
| AgRP
|
| Angiopoietin-1
| Angiopoietin-2
| ANGPTL4
| CRP (C-Reactive Protein)
| ENA-78 (CXCL5)
|
| Fas (TNFRSF6/Apo-1)
| FGF-6
| Growth Hormone
| HCC-4 (CCL16)
| IFN-gamma
|
| IGFBP-1
| IGFBP-2
| IGFBP-3
| IGF-1
| IGF-1 R
|
| IL-1 R4 (ST2)
| IL-1 R1
| IL-10
| IL-11
| IL-12 p70
|
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-6
| IL-6 R
| IL-8 (CXCL8)
|
| Insulin
| IP-10 (CXCL10)
| Leptin R
| Leptin
| LIF
|
| Lymphotactin (XCL1)
| MCP-1 (CCL2)
| MCP-3 (MARC/CCL7)
| M-CSF
| MIF
|
| MIP-1 beta (CCL4)
| MSP alpha/beta
| Osteoprotegerin (TNFRSF11B)
| Oncostatin M
| PAI-1
|
| PARC (CCL18)
| PDGF-AA
| PDGF-AB
| PDGF-BB
| RANTES (CCL5)
|
| Resistin
| SAA (Serum Amyloid A)
| SDF-1 alpha (CXCL12 alpha)
| TNF RII (TNFRSF1B)
| TNF RI (TNFRSF1A)
|
| TECK (CCL25)
| TGF beta 1
| TIMP-1
| TIMP-2
| TNF alpha
|
| VEGF-A
| XEDAR
| |||
- View Array Map
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Obesity Antibody Array G1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-AAH-ADI-G1-4.gal)
GAL (GAL-AAH-ADI-G1-8.gal)
Excel (AAH-ADI-G1_Analysis_Tool.xls)
citations
Exenatide improves hepatocyte insulin resistance induced by different regional adipose tissue
- Species:
- Human
- Sample Type:
- adipose tissue
- Year:
- 2022
- Journal Name:
- Frontiers in Endocrinology
Cell reprogramming using extracellular vesicles from differentiating stem cells into white/beige adipocytes
- Species:
- Human
- Sample Type:
- Tissue Lysate (Adipose tissue)
- Year:
- 2020
- Journal Name:
- Science advances
Depot-specific inflammation with decreased expression of ATM2 in white adipose tissues induced by high-margarine/lard intake
- Species:
- Rat
- Sample Type:
- Conditioned Media
- Year:
- 2017
- Journal Name:
- Plos One
Cancer-mediated adipose reversion promotes cancer cell migration via IL-6 and MCP-1
- Species:
- Human
- Sample Type:
- Conditioned Media (Cancer associated adipocytes and Breast normal adipocytes)
- Year:
- 2015
- Journal Name:
- Breast Cancer Research and Treatment
- PubMed ID:
- 25721605
Wang C, Gao C, Meng K, Qiao H, Wang Y (2015) Human Adipocytes Stimulate Invasion of Breast Cancer MCF-7 Cells by Secreting IGFBP-2
- Species:
- Human
- Sample Type:
- Conditioned Media (MCF-7 Cells)
- Year:
- 2015
- Journal Name:
- Plos One
- PubMed ID:
- 25747684