Orb778010-96Tests | Human Tryptophan Hydroxylase 2 (TPH2) ELISA Kit

Human Tryptophan Hydroxylase 2 (TPH2) ELISA Kit

SKU: orb778010

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Description

The Human Tryptophan Hydroxylase 2 (TPH2) ELISA Kit uses a sandwich enzyme immunoassay to quantify Tryptophan Hydroxylase 2 (TPH2) in human serum, plasma, tissue homogenates and other biological fluids. The kit has a detection range of 0.32-20 ng/mL and a sensitivity of 0.123 ng/mL.

Research Area

Metabolism Research; Pharmacology & Drug Discovery

Images & Validation

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Tested Applications	ELISA
Application Notes	standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TPH2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TPH2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TPH2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TPH2 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TPH2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TPH2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TPH2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TPH2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.123 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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NTPH ELISA Kit, Neuronal tryptophan hydroxylase ELISA Kit, Tryptophan 5-monooxygenase 2 ELISA Kit

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Human TPH2 ELISA Kit [orb563562]
Reactivity:
Human
Dynamic Range:
0.312-20ng/ml
Sensitivity:
0.188ng/ml
Sizes Available: 96 T, 48 T
Human TPH2 ELISA Kit [orb442690]
Reactivity:
Human
Dynamic Range:
0.312-20 ng/mL
Sensitivity:
0.127 ng/mL
Sizes Available: 48 T, 96 T
Human TPH2 ELISA Kit (Ready to Use) [orb552693]
Reactivity:
Human
Dynamic Range:
0.312-20 ng/mL
Sensitivity:
0.127 ng/mL
Sizes Available: 48 T, 96 T
Human Tryptophan Hydroxylase 2 (TPH2) ELISA Kit [orb2810443]
Reactivity:
Human
Dynamic Range:
0.312-20ng/mL
Sensitivity:
0.154ng/mL
Sizes Available: 48 T, 96 T, 5 x 96 T
Human Tryptophan Hydroxylase 2 (TPH2) ELISA Kit [orb1663060]
Reactivity:
Human
Sizes Available: 96 T, 48 T