IL1 beta antibody
Referentie GTX130021-100ul
Formaat : 100ul
Merk : Genetex
Neem contact op met een lokale distributeur :
Telefoonnummer : +1 850 650 7790
2
Host | Rabbit |
|---|---|
Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF |
Reactivity | Human |
| Package | 100 μl, 25 μl |
Datasheet File View product citations for antibody GTX130021 on CiteAb
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:3000 |
| ICC/IF | 1:100-1:1000 |
Not tested in other applications.
Calculated MW
31 kDa. ( Note )
Predict Reactivity
Rabbit, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human IL1 beta. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886157
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interleukin 1 beta , IL-1 , IL1-BETA , IL1F2
Cellular Localization
Secreted
Background
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq]
Database
DATA IMAGES
| | GTX130021 WB ImageUntreated (–) and treated (+) THP-1 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX130021) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
| | GTX130021 ICC/IF ImageIL1 beta antibody detects IL1 beta protein by immunofluorescent analysis.Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: IL1 beta stained by IL1 beta antibody (GTX130021) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Blue: Fluoroshield with DAPI (GTX30920). |
Application Reference
Jeong GU et al. J Virol 2023; 97 (6) : e0055623 Zika Virus Infection Induces Interleukin-1β-Mediated Inflammatory Responses by Macrophages in the Brain of an Adult Mouse Model.
X Zhang et al. Proc Natl Acad Sci U S A 2023; 120 (27) : e2300204120 Inhibition of AIM2 inflammasome activation by SOX/ORF37 promotes lytic replication of Kaposi's sarcoma-associated herpesvirus
Application : WB
Lin CY et al. Sci Adv 2023; 9 (14) : eade9944 Small-molecule PIK-93 modulates the tumor microenvironment to improve immune checkpoint blockade response.
Application : WB
Reactivity : Human
Wei KC et al. Microbiol Spectr 2023; 11 (1) : e0358622 Discrepant Activation Pattern of Inflammation and Pyroptosis Induced in Dermal Fibroblasts in Response to Dengue Virus Serotypes 1 and 2 and Nonstructural Protein 1.
Law YY et al. Aging (Albany NY) 2021; 13 (13) : 17227-17236 miR-let-7c-5p and miR-149-5p inhibit proinflammatory cytokine production in osteoarthritis and rheumatoid arthritis synovial fibroblasts.
Submit a Reference Application : WB
Reactivity : Human
REVIEW
IL1 beta antibody Cat. No. GTX130021
| Rating | | ( Average 4.5 based on 2 users reviews) |
| Western Blot(WB) | | ( Average 4.5 based on 2 users reviews) |
Application Reference
Jeong GU et al. J Virol 2023; 97 (6):e0055623 Zika Virus Infection Induces Interleukin-1β-Mediated Inflammatory Responses by Macrophages in the Brain of an Adult Mouse Model.
X Zhang et al. Proc Natl Acad Sci U S A 2023; 120 (27):e2300204120 Inhibition of AIM2 inflammasome activation by SOX/ORF37 promotes lytic replication of Kaposi's sarcoma-associated herpesvirus
Application : WB
Lin CY et al. Sci Adv 2023; 9 (14):eade9944 Small-molecule PIK-93 modulates the tumor microenvironment to improve immune checkpoint blockade response.
Application : WB
Reactivity : Human
Wei KC et al. Microbiol Spectr 2023; 11 (1):e0358622 Discrepant Activation Pattern of Inflammation and Pyroptosis Induced in Dermal Fibroblasts in Response to Dengue Virus Serotypes 1 and 2 and Nonstructural Protein 1.
Law YY et al. Aging (Albany NY) 2021; 13 (13):17227-17236 miR-let-7c-5p and miR-149-5p inhibit proinflammatory cytokine production in osteoarthritis and rheumatoid arthritis synovial fibroblasts.
Application : WB
Reactivity : Human
Tsai MH et al. Cells 2020; 9 (8) Ischemic Stroke Risk Associated with Mitochondrial Haplogroup F in the Asian Population.
Application : WB
Reactivity : Human
Nam SM et al. Nutrients 2019; 11 (1) Ascorbic Acid Mitigates D-galactose-Induced Brain Aging by Increasing Hippocampal Neurogenesis and Improving Memory Function.
Application : WB
Tokuju Okano et al. European Journal of Immunology 2018; (Epub) Porphyromonas gingivalis triggers NLRP3-mediated inflammasome activation in macrophages in a bacterial gingipains-independent manner.
Application : WB
Reactivity : Human
| SDS | |
|---|---|
| PBS.pdf | |
| Glycerol.pdf | |
| Proclin.pdf | |
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