Micro Creatinine (Cr)

Creatinine is a metabolic byproduct of muscle metabolism in the human body and is primarily excreted through glomerular filtration by the kidneys. The sources of creatinine in the blood include both endogenous and exogenous components. Serum creatinine is almost entirely filtered through the glomeruli into the primary urine and is not reabsorbed by the renal tubules. The daily production of endogenous creatinine remains almost constant, and when the intake of exogenous creatinine is strictly controlled, the serum creatinine concentration reaches a stable value. Therefore, measuring the serum creatinine concentration can reflect the glomerular filtration function.Micro Creatinine (Cr) Content Assay Kit provides a simple, convenient, and rapid method for detecting creatinine content, suitable for liquid samples such as serum, plasma, urine, etc. The principle of this assay is based on the hydrolysis of creatinine to creatine by creatinine amidohydrolase. Creatine is then converted to sarcosine and urea by creatine amidinohydrolase. Sarcosine, one of the products, is oxidized by sarcosine oxidase to generate sarcosine, hydrogen peroxide, and formaldehyde. Finally, the hydrogen peroxide reacts with chromogenic substrates 4-aminoantipyrine and N-ethyl-N-sulfopropyl-m-toluidine under the catalysis of peroxidase to produce a red quinimine compound. Since quinimine has a maximum absorption peak at a wavelength of 546 nm, within a certain concentration range, the change in absorbance at 546 nm is directly proportional to the creatinine content in the sample.

Small volumes of Cr assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9719661 is an assay kit targeting Cr. Also known as Micro Creatinine (Cr).