Mouse CD45/LCA Antibody : APC/Cy7
Referentie OASB00456
Formaat : 0.1mg
Merk : Aviva Systems Biology
Mouse CD45/LCA Antibody : APC/Cy7 (OASB00456)
| Datasheets/Manuals | Printable datasheet for Mouse CD45/LCA Antibody : APC/Cy7 (OASB00456) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | I3/2.3 |
| Isotype | IgG2b |
| Host | Rat |
| Conjugation | APC-Cyanine 7 |
| Application | FC |
| Additional Information | Description: CD45 is the common leukocyte antigen and is expressed on all cells of hematopoietic origin except erythrocytes. CD45 exists as a number of different isoforms which result from alternative RNA splicing of exons 4, 5 and 6. The I3/2.3 monoclonal antibody recognizes a framework epitope present on all CD45 isoforms. The CD45 molecule has been implicated as playing a key role in thymic development and in antigen-driven proliferation of T cells. |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | T1M1 (Thy-1-c) cells |
| Concentration | 0.1 mg/mL |
| Specificity | CD45 |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conformto characteristics of a standard reference reagent. Representative data are included in this product insert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Fluorescein and AF488 conjugates <= 1 ug/106 cells Biotin conjugate <= 1 ug/106 cells R-phycoerythrin conjugate <= 0.2 ug/106 cells R-phycoerythrin-Texas Red conjugate <= 0.2 ug/106 cells Allophycocyanin and Allophycocyanin-Cyanine 7 conjugates <= 0.2 ug/106 cells Spectral Red conjugate <= 0.2 ug/106 cells R-phycoerythrin-Cyanine 5.5 conjugate <= 0.2 ug/106 cells R-phycoerythrin-Cyanine 7 conjugate AF647 conjugate <= 0.2 ug/106 cells <= 0.2 ug/106 cells |
| Application Info | Flow cytometry 2, 5, Immunoprecipitation 2 |
| Other Applications Data | Since applications vary, you should determine the optimum working dilution forthe product that is appropriate for your specific need. |
| Other Applications Image 1 Data | Amount Used: 0.3 ug/106 cells BALB/c spleen cells were double-stained with rat anti-mouse CD45-R-PE and rat anti-mouse CD4-FITC. Small lymphocytes were gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA). |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD), R- phycoerythrin- Texas Red (R- PE- TXRD), R- phycoerythrin- Cyanine 7 (R- PECY 7), R- phycoerythrin- Cyanine 5.5 (R- PE- CY 5.5), and allophycocyanin- Cyanine 7 (APC- CY 7) conjugatesare supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS Aliquot and store at or below - 20 - The Alexa Fluor 488 (AF488) and Alexa Fluor 647 (AF647) conjugates are supplied as 0.1mg in 0.2mL ofPBS/NaN3. Store at 2- 8 C - All products should be stored at 2- 8 C. Conjugated forms should not be frozen and should be protected fromprolonged exposure to light. Each reagent is stable for the period shown on the bottle label if stored as directed. |
| Reference | 1. Johnson, P, L. Greenbaum, K. Bottomley and I. S. Trowbridge. 1989. J. Exp. Med. 169:1179 2. Haidl, I. D., D. H. W. Ng, S. Rothenberger, P. Johnson, and W. A. Jefferies. 1995. Eur. J. Immunol. 25:3370 3. Trowbridge, I.S. 1978. J. Exp. Med. 148:313 4. Trowbridge, I.S and M.L. Thomas. 1994. Ann. Rev. Immunol. 12:855. Weaver, C.T., J.T. Pingel, J.O. Nelson, and M.L. Thomas. 1991. Mol. Cell. Biol. 11(9):4415. |
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| Gene Symbol | PTPRC |
| Gene Full Name | Receptor-type tyrosine-protein phosphatase C |
| Alias Symbols | Ly-, loc, B220, Cd45, L-CA, Ly-5, Lyt-, T200, CD45R, Lyt-4 |
| NCBI Gene Id | 19264 |
| Protein Name | receptor-type tyrosine-protein phosphatase C |
| Uniprot ID | P06800 |
| Protein Accession # | NP_001104786.2 |
| Nucleotide Accession # | NM_001111316.2 |
