600200 | Mouse Leptin Clinisciences

Recombinant Active Protein

Cat. No.

600200

Unit

1 mg

Description

Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes involved in fatty acid oxidation. Severe hereditary obesity in rodents and humans is caused by defects in leptin production .In addition to its critical role in the physiologic regulation of body weight, leptin has a variety of other physiological and pathological functions resembling those of cytokines. These functions include the regulation of hematopoiesis, angiogenesis , wound healing , inflammation , and immune responses. Recombinant Mouse Leptin produced in E. coli is a single non-glycosylated polypeptide chain containing 147 amino acids with a MW of 16.1 kDa.

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Datasheet

Format

Each vial contains 1 mg lyophilized protein. Reconstitute with 1.0 ml sterile deionized water for a final concentration of 1 mg/ml.

Alternate Names

leptin; obesity factor; obese protein; LEP

Accession No.

P41160

16100 Da

Sequence

MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC

Source

E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Purity

Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by RP-HPLC calibrated against a known standard and quantitation on SDS-PAGE against a known standard.

Application Notes

Mouse Recombinant Leptin is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent stimulation of Human OB-R transfected mouse BaF/3 proliferation, is 0.52 ng/ml.

Storage

Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.

Images

For research use only, not for diagnostic or therapeutic procedures.

Mouse Leptin

Referentie 600200

Merk : Abbiotec

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