Neural Starter Kit with Midbrain/Hindbrain Neural Stem/Progenitor Cells

Rat Neural Starter Kit with Midbrain/Hindbrain Neural Stem/Progenitor Cells

Required Equipment:
1. All procedures should be conducted inside a biological safety cabinet such as a NuAire Class II Type A2.
2. The neural precursor cells can be maintained with an antibiotic if required in order to prevent bacterial contamination. All our lines have been tested with gentamycin (30ug/ml).
3. Incubator should be low oxygen capable such as the NuAire 4950 with the following settings:
a. 6% CO2
b. 2-4% O2
c. 95% humidity
d. 37°C
Note: It is best to culture neural cells in low oxygen conditions for optimal growth of rNSCs and differentiation into neurons. Atmospheric oxygen incubators may be used but will result in slower growth of rNSCs and differentiated neural cultures with lower percentage of neurons and high percentage of astrocytes and oligodendrocytes.

Required Supplies:
1. Neural StemCell Growth Medium
a. Contains Neural StemCell Growth Basal Medium and Neural StemCell Growth Supplement Pack containing the following components:
Neural StemCell Growth Base Supplement (contains glutamine)
Laminin
bFGF
EGF
Proprietary Factor
2. Neural Transition Medium to prepare NSCs for differentiation
a. Contains Neural Transition Basal Medium and Neural Transition Supplement Pack containing:
Neural Transition Base Supplement (contains glutamine)
Laminin
bFGF
EGF
3. Neural Differentiation Medium for pan-neural differentiation
a. Contains Neural Differentiation Basal Medium and Neural Differentiation Supplement Pack containing:
Neural Differentiation Base Supplement (contains glutamine)
Laminin
BDNF
GDNF

Kit Components:
1X Bottle Neural Stem Cell Growth Media
1X Bottle Neural Transition Media
1X Bottle Neural Differentiation
1X Bottle Neural Freezing Media
1X Bottle Trypsin
1X Bottle Trypsin Inhibitor
1X Vial of rat Midbrain/Hindbrain NSPCs

Storage and Stability:
Storage components as noted on protocol.