Reagentia en instrumenten voor immunologie, celbiologie en moleculaire biologie.

Phosphoinositide 3 Kinase, Recombinant, Human, p120 gamma, full length, N-terminal His-Tag (PI3K)

Afdrukken

Referentie P4073-26F-20ug

Formaat : 20ug

Merk : US Biological

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P4073-26F Phosphoinositide 3 Kinase, Recombinant, Human, p120 gamma, full length, N-terminal His-Tag (PI3K)

Swiss Prot

P48736

Grade

Purified

Accession #

AF327656

Shipping Temp

Dry Ice

Storage Temp

-70°C

Human PI3K (p120 gamma) (GenBank Accession No. AF327656), full length with N-terminal His tag, expressed in an Baculovirus infected Sf9 cell expression system (MW:~131kD).

Specific Activity:
Lot-dependent.

Sample Value:
~1200 pmol/min/ug.

Reaction:
A 50ul kinase reaction is conducted in a buffer containing 40mM Tris-HCl (pH 7.5), 20mM MgCl2, 100uM ATP, 200uM L-alpha-phosphatidylinositol/ L-alpha-phosphatidylserine (PI/PS), and 20-200ng enzyme for 15 min at 30°C. ATP reduction is detected using ADP-Glo™ Kinase Assay Platform (Promega Corporation).

Application:
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Storage and Stability:
Store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications

\Source: Baculovirus infected Sf9 cells|Purity: ≥80% by SDS-PAGE|Concentration: As reported|Form: Supplied as a liquid in 25mM Tris, pH 8.0, 69mM NaCl, 1.35mM KCl, 0.025% Tween-20, 3mM DTT, and 50% glycerol||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Form

Supplied as a liquid in 25mM Tris, pH 8.0, 69mM NaCl, 1.35mM KCl, 0.025% Tween 20, 3mM DTT, and 50% glycerol

Purity

≥80% by SDS-PAGE

References

1. Webb, LM etal., J Immunol. 175(5):2783-7 (2005). 2. Voigt, P etal., J Biol Chem. 280(6):5121-7(2005)