Pig Interleukin 6,IL-6 ELISA Kit
Referentie CSB-E06786p-96T
Formaat : 96T
Merk : Cusabio
SDS| Size | 96T,5×96T,10×96T |
Product Details
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-100 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 95-103 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| The recovery of pig IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-95 | ||||
| EDTA plasma (n=4) | 99 | 95-105 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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and FAQs
This Pig IL6 ELISA Kit was designed for the quantitative measurement of Pig IL6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.25 pg/mL-80 pg/mL and the sensitivity is 0.31 pg/mL .
Citations
- S Liu, L Wang, XK Nie, CY Song, YH Chen,SSRN,2026
- Y Song, L Feng, Y Meng, H Cheng, J Wang, Y Yue,Animals,2026
- S Liufu, W Wen, J Ouyang, B Chen, K Wang, L Xiao,Genomics,2025
- C Zhang, B Liu, Z Cui, K Wu, H Huang, Y Wang,Porcine Health Management,2025
- J Wang, H Sun, Z Peng, SQ Wang, YQ Yan, WC Luo,Antioxidants,2025
- L Ranathunga, S Abesinghe, JW Cha,Veterinary Research,2025
- X Ji, H Ding, F Zhou, F Zhang, D Wu,Ecotoxicology and Environmental Safety,2025
- MH Kim, A Subasinghe, Y Kim, HI Kwon,Emerging Microbes & Infections,2024
- H Li, R Zhang, J Qu, Y Kang, J Zhang, R Guo,Frontiers in Microbiology,2024
- M Liu,International Journal of Molecular Sciences,2024
Customer Reviews and Q&A
Sample type: Cell culture supernatant
Sample species: Pig
Sample dilution: No dilution
Review: The results are very satisfactory. I used the cell culture medium supernatant, and the other kits had interfering factors, but the CUSABIO did not, and after trying several other kits, I found that this brand was the only one that worked well.
By Anonymous
Sample type: Serum
Sample species: Pig
Sample dilution: No dilution
Review: Because of the trust in Huamei products, the pig serum was determined by Huamei kit this time, and the detection of IL-6 was not obvious. We hope to see what the situation will be if we increase the sample size.
By Anonymous
I have a pig serum sample it was centrifuged at 1500 g, for 15 min. But, the protocol of this kit CSB-E06786p suggests centrifuge at 1000 g, 15 min. I wonder to know if it would have any effects on the results.
The manual suggests centrifuge at 1000g 15min. The mentioned centrifuge at 1500 g, 15 min should affect not much theoretically. You are advised mainly to check whether there was any hemolysis or whether the centrifuge was operated at a low temperature. As you know, the temperature would raise with the speed and time during the centrifuge pr...
Target Background
(From Uniprot)
- IL-6 was low or undetectable in Boar seminal plasma.
KEGG:
STRING:
UniGene: