Streptavidin, CF®488A Conjugate
Referentie 29034
Formaat : 1MG
Merk : Biotium
Streptavidin Conjugates
A high quality biotin-binding protein conjugated to Biotium's signature bright and photostable fluorescent CF® Dyes, and a selection of other labels.
Jurkat cells were stained with CD3-biotin followed by streptavidin-APC (pink), and detected in the R660 (APC) channel. Unstained cells (black) and no-primary control cells (gray) are also shown. 
Jurkat cells were stained with CD3-biotin followed by streptavidin-PerCP (magenta), and detected in the B690 (PerCP) channel. Unstained cells (black) and no-primary control cells (gray) are also shown. 
Lymphocyte-gated PBMCs were stained with CD3-biotin followed by streptavidin-CF®488A (green), and detected in the B525 (FITC) channel. Unstained cells (black) and no-primary control cells (gray) are also shown. 
MCF-7 cells were stained with CD9-biotin followed by streptavidin-PE (orange), and detected in the Y585 (PE) channel. Unstained cells (black) and no-primary control cells (gray) are also shown. Product Description
CF® Dyes conjugates of Streptavidin are high quality biotin-binding proteins labeled with the superior CF® Dyes or a selection of other labels. These conjugates are typically used as secondary reagents to detect biotinylated probes such as primary antibodies for flow cytometry, western blotting, immunofluorescence staining, and other applications.
- Conjugates with wide choice of CF® Dye colors, plus HRP, R-PE, APC, PerCP, and Alkaline Phosphatase
- Superior CF® Dyes are bright, photostable, and water-soluble
- Ideal for flow cytometry, western blotting, and immunofluorescence staining
Blue dyes have lower fluorescence and can give higher non-specific background than other dye colors. Conjugates of blue fluorescent dyes like CF®350, CF®405S, and CF®405M are not recommended for detecting low abundance targets.
Superior CF® Dyes
Biotium’s next-generation CF® Dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.
Streptavidin Conjugates
| Product | Conjugation | Ex/Em | Size | Catalog number |
|---|---|---|---|---|
| CF®350 Streptavidin | CF®350 | 347/448 nm | 1 mg | 29031 |
| CF®405S Streptavidin | CF®405S | 404/431 nm | 1 mg | 29032 |
| CF®405M Streptavidin | CF®405M | 408/452 nm | 1 mg | 29033 |
| CF®405L Streptavidin | CF®405L | 395/545 nm | 1 mg | 29056 |
| CF®430 Streptavidin | CF®430 | 426/498 nm | 1 mg | 29065 |
| CF®440 Streptavidin | CF®440 | 440/515 nm | 1 mg | 29066 |
| CF®488A Streptavidin | CF®488A | 490/515 nm | 1 mg | 29034 |
| CF®514 Streptavidin | CF®514 | 516/548 nm | 1 mg | 29081 |
| CF®532 Streptavidin | CF®532 | 527/558 nm | 1 mg | 29030 |
| CF®543 Streptavidin | CF®543 | 541/560 nm | 1 mg | 29043 |
| CF®555 Streptavidin | CF®555 | 555/565 nm | 1 mg | 29038 |
| CF®568 Streptavidin | CF®568 | 562/583 nm | 1 mg | 29035 |
| CF®583R Streptavidin | CF®583R | 586/609 nm | 1 mg | 29086 |
| CF®594 Streptavidin | CF®594 | 593/614 nm | 1 mg | 29036 |
| CF®633 Streptavidin | CF®633 | 630/650 nm | 1 mg | 29037 |
| CF®640R Streptavidin | CF®640R | 642/662 nm | 1 mg | 29041 |
| CF®647 Streptavidin | CF®647 | 650/665 nm | 1 mg | 29039 |
| CF®660R Streptavidin | CF®660R | 663/682 nm | 1 mg | 29040 |
| CF®680R Streptavidin | CF®680R | 680/701 nm | 1 mg | 29072 |
| CF®740 Streptavidin | CF®740 | 742/767 nm | 1 mg | 29129 |
| Alkaline Phosphatase Streptavidin (1 mg/mL) | Alkaline Phosphatase | N/A | 100 uL | 29071-100uL |
| 1 mL | 29071-1mL | |||
| HRP Streptavidin | HRP | N/A | 1 mg | 29049 |
| R-PE Streptavidin (0.5 mg/mL) | R-PE | 496, 546, 565/578 nm | 200 uL | 29044-200uL |
| 1 mL | 29044-1mL | |||
| APC Streptavidin (0.5 mg/mL) | APC | 650/660 nm | 200 uL | 29048-200uL |
| 1 mL | 29048-1mL | |||
| PerCP Streptavidin (0.5 mg/mL) | PerCP | 482/675 nm | 200 uL | 29140-200uL |
| 1 mL | 29140-1mL |
Storage and Handling
Liquid formats:
Streptavidin, R-PE Conjugate and PerCP Conjugate: Store at 4°C, protected from light. Storage at -20°C is not recommended for R-PE or PerCP conjugates. Product is stable for at least 6 months from date of receipt when stored as recommended.
Streptavidin, APC Conjugate: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended.
Streptavidin, Alkaline Phosphatase Conjugate: Store at -20°C. Product is stable for at least 6 months from date of receipt when stored as recommended.
Lyophilized format:
CF® Dye Streptavidin Conjugates: Product is stable for at least 2 years at -20°C with desiccant. Add 0.5 mL of PBS to 1 mg lyophilized CF® Dye Streptavidin Conjugate and mix gently to dissolve.
Streptavidin, HRP Conjugate: add 1 mL pH ~7.4 1X PBS and mix gently to dissolve. Reconstituted HRP conjugate can be stored in aliquots at -20°C for at least 6 months. Do not add azide to HRP conjugates.
Technology
Tech Tips
Product Attributes
Colors
Detection method/readout
Antibody reactivity (target)
Antibody/conjugate formulation
Product origin
Documents, Protocols, SDS and COA
SDS
Datasheet
COA
Request the Certificate of AnalysisReferences
Download a list of CF® dye references.
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