Tissue, Western Blot, Human Adult Normal, Blot IV, heart, brain, kidney, liver, lung, pancreas, spleen, skeletal muscle, stomach, small intestine, colon, rectum, uterus, prostate, testis, placenta HisTek™

Protein Western Blots are manufactured by using high-quality protein isolated from documented tissues. The tissue is excised, immediately frozen, and then pathologically identified. Protein is isolated by proprietary techniques. Each lane of the blot contains 50ug of protein.

The protein is separated by 4-20% gradient gel electrophoresis and transferred to a PVDF membrane. Human placenta protein is used as an interblot control lane for protein expression comparison between different blots.

Protein Western Blots are supplied ready for immediate use, so that you can immediately screen for your protein of interest without the hassle of obtaining hard to find tissues and processing the protein yourself.

Features:
Ready to use, pre-blotted with high quality protein
Suitable for both radioactive and non-radioactive probes
Extensive selection of blots
Documentation of tissues' clinical histories is available (additional cost)
“Matched" tissue slides or cDNA might be available for immunohistochemistry, in situ hybridization or PCR
High sensitivity and low background
Normalized based on amount of protein used in blots
Interblot control built into every blot

Applications:
Identification of normal, tumor or disease tissues specific gene expression
Protein size determination
Gene expression pattern analysis
Compare expression level of novel genes
Examining alternative splicing and prematur etermination of specific proteins

Tissues on Blots (different tissues, different single donors)
lane 1: heart
lane 2: brain
lane 3: kidney
lane 4: liver
lane 5: lung
lane 6: pancreas
lane 7: spleen
lane 8: skeletal muscle
lane 9: stomach
lane 10: small intestine
lane 11: colon
lane 12: rectum
lane 13: uterus
lane 14: prostate
lane 15: testis
lane 16: placenta

Storage Conditions:
Store blots at 4°C. Store moist in a sealed bag. Keep away from light.

Note: USBio recommends following protocol provided. Has not been evaluated for compatibility with other blocking and stripping methodologies or reagents.